Immobilized laccase-based biosensor for the detection of disubstituted methyl and methoxy phenols – application of Box–Behnken design with response surface methodology for modeling and optimization of performance parameters

Abstract

An amperometric principle-based biosensor, employing immobilized laccase enzyme from Trametes versicolor, was developed for the detection of disubstituted methyl and methoxy phenols. Three immobilization methods such as entrapment, cross-linking, and co-cross-linking, with bovine serum albumin (BSA) on nylon membrane have been compared. Among tested methods of immobilization, co-cross-linking method with BSA was superior to the other methods in terms of; sensitivity, limit of detection, response time, and operating stability. The increased sensitivity of the probe optimization of concentrations of laccase, BSA and glutaraldehyde can be achieved by, employing the Box–Behnken design of experiment.

Keywords:

• Amperometric
• bovine serum albumin
• co-cross-linking
• nylon

Acknowledgements

C. Sarika gratefully acknowledges the Junior Research Fellowship from Department of Science and Technology, Govt. of India. The authors acknowledge the support from Mr. Lokesh, Assistant Professor, Department of Biotechnology Engineering, MSRIT, Bangalore.

Declaration of interest

The present work was supported by the project fund “DST/TSG/ME/2009/65” from Department of Science and Technology, Govt. of India.