Antisense oligonucleotide delivery to cancer cell lines for the treatment of different cancer types

Abstract

Amphiphilic poly(3-hydroxylalkanoate) (PHA) copolymers find interesting applications in drug delivery. The aim of this study was to prepare nucleic acid adsorbed on (PHB-b-PEG-NH₂) nanoparticle platform for gene delivery. For this purpose, PHB-b-PEG-NH₂ block copolymers were synthesized via transesterification reactions. The copolymers obtained were characterized by Proton Nuclear Magnetic Resonance (¹H-NMR), Fourier Transform Infrared Spectrometer (FTIR), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) techniques. The cytotoxic, apoptotic and necrotic effects of these nanoparticles in the MDA 231 human breast cancer cell, the A549 human lung cancer cell and the L929 fibroblast cell lines were also investigated.

Keywords:

• Antisense oligonucleotide
• fibroblast cells
• human breast cancer cells
• human lung cancer cells
• PHB-PEG block copolymer
• PHB-PEG nanoparticle

Acknowledgements

The authors thank Tuğba Toraman for her contributions as regards to copolymer preparation stage and Evren Sean Kilicay for editing the article.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

This work was financially supported by the Bulent Ecevit University Research Fund (Grant no. BEU-2012-M6-00-03).