Effects of antioxidants, catalase and α-tocopherol on cell viability and oxidative stress variables in frozen-thawed mice spermatogonial stem cells

Abstract

Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P ≤ 0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the antioxidant groups. ROS production with antioxidant was significantly lower compared with the control group. Cryopreservation with the addition of the antioxidants can help increase the number of SSCs and improve the quality and viability of these cells after cryopreservation.

Keywords:

• Catalase
• cryopreservation
• spermatogonial stem cell
• α-tocopherol

Declaration of interest

The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work. There were also no conflict of interest for designing and carrying out this current work. The authors alone are responsible for the content and writing of this article.

Funding information

We all want to say that this is an original article, which has been supported by Tehran University of Medical Sciences with the Grant No. 20163. We are grateful for the funding support by the University. The results described in this article were part of student thesis. In the present work, we used animal model with considering all the rights based on Ethical Committee of Medical Faculty of Tehran University.