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Original Articles

Green synthesis of silver nanoparticles using Rheum palmatum root extract and their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa

, , , , &
Pages 372-379 | Received 07 Oct 2015, Accepted 27 Feb 2016, Published online: 29 Mar 2016
 

Abstract

In the present study, silver nanoparticles (AgNPs) were synthesized by using aqueous root extracts of Rheum palmatum and characterized by various spectroscopic methods. The nanoparticles were found to be in hexagonal and spherical shapes. The average particle size was found to be 121 ± 2 nm with zeta potential values of −21.6 mv by dynamic light scattering (DLS) method. Gas chromatography–mass spectrometry (GC-MS) analysis of R. palmatum revealed 35 compounds. The synthesized AgNPs showed significant activity against Staphylococcus aureus and Pseudomonas aeruginosa with IC90 values of 15 μg/ml and IC50 values of 7.5 μg/ml, respectively. The protein leakage level was high and morphological changes occurred in bacteria treated with AgNPs.

Acknowledgements

We acknowledge Chang Hyun Lee, GBST, Seoul National University for his technical assistance.

Disclosure statement

The authors report no declaration of interest. The authors alone are responsible for the content and writing of the paper.

Funding information

The authors would like to acknowledge their sincere appreciation to National Institute of Animal Science, RDA for its funding through the Research project NO (PJ010168).

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