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Abstract
The influence of incubation time and temperature on the nitroblue tetrazolium (NBT) test in neutrophils from 40 patients with bacterial infections and 40 healthy individuals has been examined. Incubation time and temperature strongly influenced the reduction of NBT to formazan by patient neutrophils. Incubation of the blood-NBT mixture at 37°C for 15 min provided optimum differences between results in patients and controls. Standardization of the incubation procedure at 37°C and 15 min, using water bath incubation for accurate temperature control, is recommended.