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Abstract
A method for the demonstration of the effect of normal human serum on L-phase variants of Staphylococcus aureus on solid medium is presented. Hypertonicity of the medium is achieved by adding sucrose and sodium chloride in about equimolal amounts. Agarose with addition of DEAE-dextran were used to obtain a solid medium. Normal human and rabbit serum were found to kill L-phase variants of 5 Staph. aureus strains on this medium. Heat inactivation of the sera abolished most of the L-phase variant killing ability.