DNA Content of Micronuclei in Human Lymphocytes

Madeleine Pincu Department of Radiological Sciences, University of California, Los Angeles, California 90024, U.S.A.

Hannes Callisen Department of Radiation Oncology, University of California, Los Angeles, California 90024, U.S.A.

Amos Norman Department of Laboratory of Biomedical, University of California, Los Angeles, California 90024, U.S.A.; Department of Environmental Sciences, University of California, Los Angeles, California 90024, U.S.A.

Abstract

We have calculated the distribution of DNA contents in micronuclei (MN) induced by ionizing radiation in human lymphocytes on two assumptions: the MN arise from acentric chromosome fragments (ACF), and the ACF result from the random breakage and rejoining of chromosomes. Measurements show that about 80 per cent of MN have a DNA content in the range of 0·5-6 per cent of the G1 nucleus. This group is consistent with the model and shows little dependence on radiation dose over the dose range of 0·5-4 Gy, or on lymphocyte culture time, varying from 48 to 76 hours. The MN with DNA content from 6 to 20 per cent of the G1 nucleus are probably the result both of spindle defects and of DNA synthesis in MN.

Keywords

Micronuclei, Dna, Human Lymphocytes, Ionizing Radiation

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DNA Content of Micronuclei in Human Lymphocytes

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DNA Content of Micronuclei in Human Lymphocytes

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