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Abstract
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.
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Acknowledgements
This work was supported by a Wellcome Trust Programme Grant (GR079862), Wellcome Trust Technology Development Grant (GR087730) and MRC Programme Grant (MR/J003360/1), and by the Oxford Partnership Comprehensive Biomedical Research Centre (http://www.oxfordbrc.org), with funding from the Department of Health's National Institute for Health Research Biomedical Research Centres funding scheme. The views expressed in this publication are those of the authors and not necessarily those of the Department of Health.