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Technical Reports

Potentials and capabilities of the Extracellular Vesicle (EV) Array

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Article: 26048 | Received 18 Sep 2014, Accepted 03 Nov 2015, Published online: 08 Apr 2015
 

Abstract

Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.

Acknowledgements

This work was supported by Toyota Fonden, Det Obelske Fami-liefond and Aalborg Frivillige Bloddonorers Fond.