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Does Vitamin E or Vitamin E plus Selenium improve reproductive performance of rams during hot weather?

Può la Vitamina E, da sola o con Selenio, migliorare le prestazioni riproduttive degli arieti durante la stagione calda?

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Pages 743-754 | Received 23 Sep 2008, Accepted 19 Mar 2009, Published online: 01 Mar 2016
 

Abstract

The objective of this study was to determine the effect of Vitamin E (Vit E) and Selenium (Se) on semen quality, sexual activity, packed cell volume (PCV), and white blood cell counts (WBC) of Awassi rams during the hot season.

Twelve Awassi rams were subdivided into three groups and treated for 90 days. Rams in the 1st group (T1) were treated twice weekly with 175 mg/ram vitamin E at intervals of 12h; rams in the 2nd group (T2) were treated on the same schedule with 70 mg/ram vitamin E plus 2800 mg selenium; and rams in 3rd group (T3) served as controls. Sperm quality (percentage of motile cells and percentage of morphologically normal cells) and quantity (sperm volume, and concentration) were recorded weekly. Sexual activity was tested by using the pen libido test at monthly intervals. Blood samples were taken before treatment and after the 1st, 2nd, and 3rd months. Semen quality was significantly affected by treatments: the ejaculate volume increased in T2 vs T1, and T3; mass activity and individual motility were increased in T1 and T2 vs T3. Sperm concentration was increased in T2 and T1 vs T3, and the percentage of dead and abnormal spermatozoa was reduced in T1 and T2 vs T3, though the differences were not statistically significant. Pen libido test showed reduced reaction time for the first mount in the 1st month in T2 and T1 vs T3. The number of serves was increased in groups T1 and T2 vs T3. There were no significant differences in PCV among groups. After 3 months from the beginning of treatments, the percentage of lymphocytes increased in T1 and T2 vs. T3, while the percentage of neutrophils was reduced in T2 vs T3 in the 2nd and 3rd months.

The results of this experiment indicate that treatments with vitamin E alone or in combination with selenium improved semen characteristics and reproductive performance of Awassi rams during the hot season.

Riassunto

Lo scopo di questo studio é stato quello di determinare l’effetto di Vitamina E (Vit E) e Selenio (Se) su qualità del seme, attività sessuale, valore ematocrito (PCV) e conteggio di leucociti (WBC) in arieti Awassi durante la stagione calda.

Dodici arieti Awassi vennero suddivisi in tre gruppi e sottoposti a trattamento per 90 giorni. Gli arieti del 1° gruppo (T1) furono trattati due volte alla settimana con 175 mg/ariete di vitamina E con un intervallo di 12h, gli arieti del 2° gruppo (T2) furono trattati allo stesso modo con 70 mg/ariete di vitamina E e 2800 mg di selenio, mentre gli arieti del 3° gruppo (T3) servirono da controllo. La qualità del seme (percentuale di cellule mobili e percentuale di cellule morfologicamente normali) e la quantità (volume e concentrazione spermatica) vennero registrate settimanalmente. L’attività sessuale venne valutata tramite “pen libido test” a cadenza mensile. Campioni di sangue vennero prelevati prima del trattamento e dopo il 1°, 2° e 3° mese. La qualità del seme venne significativamente influenzata dai trattamenti, con incremento del volume di eiaculato in T2 vs T1 e T3, di attività massale e di motilità individuale in T1 e T2 vs T3. La concentrazione spermatica incrementò in T2 e T1 vs T3, e la percentuale di spermatozoi morti e anormali si ridusse in T1 e T2 vs T3, sebbene le differenze non siano risultate statisticamente significative. Il “pen libido test” mostrò un ridotto tempo di reazione alla prima monta nel 1° mese in T2 e T1 vs T3. Il numero di accoppiamenti incrementò nei gruppi T1 e T2 vs T3. Non vi furono significative differenze tra i gruppi per quanto concerne il PCV. Dopo 3 mesi di trattamento, la percentuale di linfociti risultò aumentata in T1 e T2 vs T3, mentre la percentuale di neutrofili si ridusse in T2 vs T3 nel 2° e 3° mese.

I risultati di questo esperimento indicano che i trattamenti con vitamina E, da sola o in combinazione con selenio, migliorano le caratteristiche del seme e la performance riproduttiva di arieti Awassi durante la stagione calda.

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