64
Views
1
CrossRef citations to date
0
Altmetric
Article

Genotype at the MTNR1A locus and response to melatonin treatment in Sarda lambs

, , , &
Pages 114-116 | Published online: 07 Mar 2016
 

Abstract

With the aim to evaluate the effect of melatonin treatment and melatonin receptor 1A (MTNR1A) genotype on advance of puberty, 423 Sarda lambs were chosen. On June 26th, they were divided into three groups, each of 141 animals (groups 0, 1, and 2), on the basis of live weight. On June 30th, animals in group 1 received a single implant (18 mg melatonin), while group 2 received two implants. Group 0 was untreated. Thirty-five days after treatment (August 4th), rams were introduced and after 40 days they were removed. From January 1st to February 10th lambing dates were recorded. Genomic DNA was extracted and subjected to PCR for the amplification of exon II and then digested with enzymes MnlI and RsaI and placed into +/+, +/., or ./. group for MnlI and C/C, C/T, or T/T group for RsaI. Samples were cloned and sequenced. Data obtained were subjected to χ2 test in order to evaluate the difference in fertility among groups and the link between genotype and reproductive activity. Genotype +/+ and C/C showed the highest incidence. Treated groups showed a higher number of lambing at 10th February compared to control group (P<0.04). Melatonin treatment results more efficient in +/+ genotype.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.