Effect of in vitro exposure to cadmiumand copper on sea bass blood cells

Abstract

Blood cells freshly collected from sea bass (Dicentrarchus labrax) were exposed in vitro to different concentrations of cadmium (Cd) and copper (Cu) at 10-⁷ M, 10-⁵ M, 10-³ M, and examined for neutral red retention capacity and for cell vitality with MTT assay. A relationship between heavy metal exposure and alteration in responses of blood cells in a dose-time-dependent was found. Our results showed that fish blood cells may constitute an interesting biological model for experimental and applied toxicology, especially in the case of environmental pollution.

Key words:

• Fish blood cells
• Heavy metals
• NR
• MTT