Strategies and Analytical Workflows to Extend the Dynamic Range in Quantitative LC–MS/MS Analysis

Abstract

Aim: To evaluate alternative analytical strategies to extend the dynamic range in quantitative LC–MS/MS. Methods & results: Two approaches based on prior or no prior knowledge of expected exposure levels were evaluated. These approaches make use of two analytical strategies, which include the use of more than one injection volume or dilution of sample extract with solvents or solvent mixtures. A total of 16 compounds with varying logP values were classified into polar and nonpolar groups and used in this evaluation. From the two analytical strategies, three workflows were derived. Conclusion: All three workflows were successfully evaluated and resulted in good accuracy (80–120%) for all the compound groups.

Keywords:

• AQL approaches
• bioanalysis
• dilution
• dynamic range
• injection volume
• LC–MS
• nanovalve
• orders of magnitude
• quantitation
• reanalysis

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline/doi/suppl/10.4155/bio-2018-0309

Financial & competing interests disclosure

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline/doi/suppl/10.4155/bio-2018-0309