Abstract
Aim: Study was designed to develop ultra-fast liquid chromatography method of antitubercular compound S006–830 in rat intestinal perfusate and apply method for determination of effective permeability coefficient (Peff), absorption rate (Ka) and role of P-glycoprotein (P-gp) in the absorption. Methods: Chromatography was performed. Peff, Ka of S006–830, role of P-gp in the absorption of S006–830 were determined by single-pass intestinal perfusion studies. Results: Method was found sensitive, reproducible. Recoveries of S006–830 were found around 60%. Method was applied for determination of Peff and Ka. Peff and Ka values indicated that S006–830 possesses high permeability and high absorption rate. Conclusion: Co-infusion of S006–830 with verapamil in single pass intestinal perfusion study demonstrated that P-gp do not have any significant role in the absorption of S006–830.
Acknowledgements
The authors acknowledge the Director, CSIR-CDRI and Council for Scientific and Industrial Research (CSIR) for availing all the facilities required for this research work.
Financial & competing interests disclosure
The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.
No writing assistance was utilized in the production of this manuscript.
Ethical conduct of research
The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.