Expression of non-symbiotic hemoglobin 1 and 2 genes in rice (Oryza sativa) embryonic organs

Abstract

Rice (Oryza sativa) contains five copies of the non-symbiotic hemoglobin (hb) gene, namely hb1 to hb5. Previous analysis by RT-PCR revealed that rice hb1 expresses in roots and leaves and hb2 expresses in leaves. However, it is not known whether or not hb1 and hb2 express in rice embryonic organs. Here, we report the expression of hb1 and hb2 genes in rice embryonic organs using RT-PCR and specific oligos for Hb1 and Hb2. Our results indicate that hb1 and hb2 genes express in embryonic organs in rice growing under normal conditions. Specifically, hb1 expresses in rice embryos and seminal roots, and hb2 expresses in embryos, coleoptiles and seminal roots. These observations suggest that Hb1 and Hb2 coexist and function in rice embryonic organs.

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Acknowledgements

Authors wish to express their gratitude to Miss Gillian Klucas and Dr. Gautam Sarath for English corrections and useful comments to improve the contents of this addendum.

Figures and Tables

Figure 1 Expression analysis of hb1 and hb2 genes in rice embryonic organs. Amplification of the rice Hb1 and Hb2 transcripts was performed by RT-PCR using specific oligos for Hb1 and Hb2 and conditions described by Arredondo-Peter et al.8

Table 1 Expression of nshb genes in rice organs

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