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Abstract
Localization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that revealing novel biological insights is not. Here, we outline why data analysis and the design of robust test samples may hold the key to harness the full potential of localization microscopy.
Acknowledgments
We acknowledge funding from the Australian Research Council (D.M.O., K.G.), National Health and Medical Research Council of Australia (K.G.), Deutsche Forschungsgesellschaft, Grant SA829/8-1 (M.S.) and Human Frontier Science Program (K.G.).