Septin filament organization in Saccharomyces cerevisiae

Abstract

Septins are a family of GTP-binding, membrane-interacting cytoskeletal proteins, highly conserved and essential in all eukaryotes (with the exception of plants). Septins play important roles in a number of cellular events that involve membrane remodeling and compartmentalization. One such event is cytokinesis, the last stage of cell division. While cytokinesis is ultimately achieved via the mechanical contraction of an actomyosin ring at the septum, determination of the location where cytokinesis will take place, and recruitment of factors involved in signaling events leading to septation requires the activity of septins. We are working towards dissecting the properties of septins from the budding yeast Saccharomyces cerevisiae, where they were first discovered as cell cycle mutants. In our studies we have employed several complementary electron microscopy techniques to describe the organization and structure of septins both in vitro and in situ.

Keywords: :

• septins
• cytokinesis
• bud neck filaments
• electron tomography
• cytoskeleton

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Acknowledgments

The authors were funded by a Jane Coffin Childs Postdoctoral Research Fellowship (fellowship 61-1357 to A.B.) and by the Howard Hughes Medical Institute (E.N.).