Dynamic interactions of Sup35p and PrP prion protein domains modulate aggregate nucleation and seeding

Abstract

The mouse monoclonal antibody POM2 directed against the PrP octapeptide region was a generous gift of Dr. Polymenidou and Dr. A. Aguzzi (Institute of Neuropathology, University of Zürich). The plasmid pEGFP-HD72Q was kindly provided by Dr. E. Wanker (Max Delbrück Center of Molecular Medicine, Berlin). We thank members of the Vorberg and Schätzl laboratories for constructive comments on this work. Financial support for this work was provided by the Deutsche Forschungsgemeinschaft SFB 596 B14, VO 1277/1-2, the European Commission (grant TSEUR LSHB-CT-2005-018805) and by the EU NoE Neuroprion.