Differences in early callose deposition during adapted and non-adapted powdery mildew infection of resistant Arabidopsis lines

Abstract

The deposition of callose, a (1,3)-β-glucan cell wall polymer, can play an essential role in the defense response to invading pathogens. We could recently show that Arabidopsis thaliana lines with an overexpression of the callose synthase gene PMR4 gained complete penetration resistance to the adapted powdery mildew Golovinomyces cichoracearum and the non-adapted powdery mildew Blumeria graminis f. sp hordei. The penetration resistance is based on the transport of the callose synthase PMR4 to the site of attempted fungal penetration and the subsequent formation of enlarged callose deposits. The deposits differed in their total diameter comparing both types of powdery mildew infection. In this study, further characterization of these callose deposits revealed that size differences were especially pronounced in the core region of the deposits. This suggests that specific, pathogen-dependent factors exist, which might regulate callose synthase transport to the core region of forming deposits.

Keywords: :

• (1,3)-β-glucan
• Arabidopsis thaliana
• AtGSL5
• PMR4
• callose synthase
• cell wall
• papillae
• plant defense
• powdery mildew

Disclosure of Potential Conflicts of Interest

No potential conflicts of interest were disclosed.

Acknowledgments

Funding was provided in part by a postdoctoral research fellowship from the Deutsche Forschungsgemeinschaft (C.A.V.), the German Federal Ministry of Education and Research (BMBF, FKZ 0315521A, C.A.V., M.N.), the Carnegie Institution of Science (S.C.S.) and the Energy Biosciences Institute (S.C.S. and C.A.V.).