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Articles

Mycoflora of Chicken-Viscera with Aid of RAPD Technique as a Tool for Confirmation

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Pages 5-12 | Received 09 Jan 2002, Published online: 22 Jun 2018
 

Abstract

A total of 100 samples of chicken-viscera were collected from different poultry-slaughtering houses in Austria; (20 samples of each of gizzard, heart, intestine, liver and spleen). Intestine and gizzard were heavy contaminated with moulds than other examined visceral organs (4.4×05 and 2.6×104 colonies/1 g of the samples, respectively). Fungal contamination was not detected in all samples of heart and spleen. Eighty-five mould isolates were collected from the examined samples, the majority of isolates belonging to Aspergillus glaucus group (20.0%) and Trichoderma (14.1%). These isolates comprised 15 species belonging to 9 genera. Members of Aspergillus glaucus (telomorph: Eurotium) group and Trichoderma were further confirmed their identification using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique.

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