Comparison of Characteristics of Ganoderma lucidum According to Geographical Origins (III): Classification between Species of Genus Ganoderma Using Dikaryon- Monokaryon Mating

Abstract

A Monokaryotic strain G8M without clamp connections was isolated from germinated basidiospore that was obtained from cultivated fruit body. Strain G8M was used as a tester isolate for ‘dikary on-mono kary on mating’ (di-mon mating) with the strains of Ganoderma lucidum, G6 and G35 (Korean wild strains), G3 (Taiwan), G4 (Canada), G15 (America), G. oregonense G24, G. resinaceum G28, G. oerstedii G23, and G. subamboinense G29. Isolate G8M was compatible to Korean strains G6 and G35, but was incompatible to foreign strains G3, G4, or G15. Compatible reactions between strains were readily observed macroscopically. Clear barrage lines formed between incompatible strains. These clear lines were not apparent in compatible di-mon matings. The Korean strains were morphologically distinct; they did not form any chlamydospores, and stopped growth at 35^oC. The strains of G. lucidum from Korea may be considered as different species from Taiwan, Canadian and American cultures.

Key Words:

• Classification
• Compatibility
• Di-mon mating
• Ganoderma lucidum