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Abstract
Species of Phellinus were known to harmful fungi causing white pocket rot and severe plant disease such as canker or heart- rot in living trees in the West, but some species have been used to traditional medicines in the Orient for a long time. In this study the partial D1-D2 nucleotide sequences of 28S ribosomal DNA from 13 Phellinus strains were determined and compared with the sequences of 21 strains obtained from GenBank database. According to the neighbor-joining (NJ) method comparing the sequence data the phylogenetic tree was constructed. The phylogenetic tree displayed the presence of four groups. Group I includes P. ferreus, P gilvus and P johnsonianus, Group II contains P laevigatus, P. conchatus and P. trem- ulae, Group III possesses P. linteus, P. weirianus, P. baumii, P. rhabarbarinus and P igniarius, and Group IV comprises P. pini, P chrysoloma. P linteus and P baumii, which were used mainly in traditional medicine, belong to the same group, but exactly speaking both were split into two different subgroups. To detect P linteus only, we developed the PCR primer, D12HR. The primer showed the specific amplification of P linteus, which is permitted to medicinal mushroom in the East. The results make a potential to be incorporated in a PCR identification system that could be used for the rapid identification of this species from its related species, P linteus especially.
