Estimation of Cell Concentration by Light Transmitter During the Culture of Methylotrophic Yeast Pichia pastoris

Abstract

The multiple correlation coefficient between the values determined by dry weight and those determined by fluorometer was observed with r = 0.96 and the standard error of calibration was 0.034. Using the best calibration data, in order to reconfirm the reliability of the fluorometer results in comparison with those obtained by dry weight on the cell concentration, fed-batch cultures were carried out. The results obtained by fluorometer measurements were in good agreement with those obtained by dry weight. The on-line monitoring of cell concentration by the fermentor system linked to a computer equipped with fluorometer was successfully carried out.

Keywords::

• Fluorometer system
• Light transmitter
• Pichia pastoris