Molecular Marker Related to Fruitbody Color of Flammulina velutipes

Abstract

White and brown strains of Flammulina velutipes were inter-crossed. All F₁ showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.

Keywords::

• Backcross
• Bulked segregant analysis
• Flammulina velutipes
• Molecular marker