A New Technique for Single Spore Isolation of Two Predacious Fungi Forming Constricting Ring

Abstract

A new technique for single spore isolation was developed for predacious fungi forming constricting rings directly on the spores using Dactylaria brochopaga and Arthrobotrys dactyloides. Constricting rings were induced directly on the spores by transferring the spores in 25 ppm solution of DL-Valine in sterile distilled water. Freshly hatched and thoroughly washed second stage juveniles of Meloidogyne incognita were transferred into cavity blocks containing induced rings for trapping and killing of nematodes. The killed nematodes were surface sterilized with streptomycin and inoculated into petri dishes containing maize meal agar media with 100 ppm streptomycin. The petri dishes were incubated at 29±1°C for few days which yielded axenic culture of these fungi.

Key Words:

• Arthrobotrys dactyloides
• Constricting ring
• DL-Valine
• Dactylaria brochopaga
• Meloidogyne incognita
• Predacious fungi