Comparison of RAPD, AFLP, and EF-1 α Sequences for the Phylogenetic Analysis of Fusarium oxysporum and Its formae speciales in Korea

Abstract

Although Fursarium oxysporum causes diseases in economically important plant hosts, identification of F. oxysporum formae speciales has been difficult due to confusing phenotypic classification systems. To resolve these complexity, we evaluated genetic relationship of nine formae speciales of oxysporum with random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and translation elongation factor-1 alpha (EF-1α) gene. In addition, the correlation between myeotoxin content of fusaric acid and isolates based on molecular marker data was evaluated using the modified Mantel’s test. According to these result, these fusaric acid-producing strains could not identify clearly, and independent of geographic locations and host specificities. However, in the identification of F. oxysporum formae speciales, especially, AFLP analysis showed a higher discriminatory power than that of a the RAPD and EF-1α analyses, all three techniques were able to detect genetic variability among oxysporum formae speciales in this study.

Keywords::

• Amplified fragment length polymorphism
• Fusarium oxysporum
• Genetic variability
• Random amplified polymorphic DNA
• Translation elongation factor-1 alpha