Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

Abstract

Flammulina velutipes was transformed efficiently’ by Agrobacterium-medmted transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.

Keywords::

• Agrobacterium
• Flammulina velutipes
• Gene tagging
• Marker inheritance
• Transformation