Development of Detection Methods for Cellulolytic Activity of Auricularia auriculajudae

Abstract

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35°C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25°C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.

Keywords::

• Auricularia auricula-judae
• Cellulolytic enzyme
• Chromogenic media
• Congo red