Native and Recombinant Fatty Acid Binding Protein 3 from Fasciola Hepatica as a Potential Antigen

ABSTRACT

Lipid-binding proteins are members of widely distributed protein family. They take part in metabolism of different lipophilic ligands and also have a role in physiological activity, metabolism and disposition of essential hydrophobic compounds (fatty acids, phospholipids, eicosanoids, retinoids, etc.) which are important molecules involved in several cellular processes including gene transcription, immune responses etc. Fatty acid binding proteins (FABPs) are mostly cytosolic but several members are excretory-secretory (E-S) proteins. There is a lot of data that helminthic FABPs from E/S products cause antigenic and allergenic reactions.

The aim of the present study is to make a detailed investigation of native and produced recombinant FABP3 as a potential antigen for vaccine creation. The native FABP3 from Fasciola hepatica was purified and recombinant one was expressed in E. coli. Polyclonal antibody was produced against recombinant FABP3 and it was tested on the native one. Obtained results give the opportunity to investigate the effectiveness of the native and recombinant FABP3 as an immunotherapeutic agent and if it could be used for immunoprophilactic.

Keywords:

• Fasciola hepatica
• antigens
• alternative vaccines
• fatty acid binding protein 3