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ABSTRACT
Two natural β-carboline alkaloids were isolated from the culture filtrate of Thermoactinomyces sp. imbas-14 strain. The organism was isolated from penguin excrements collected on the Antarctic Livingston Island. Based on the effect of temperature on its growth rate, the strain is thermophilic with an optimal growth temperature at 50°C. The compounds were purified by solvent extraction, silica gel column chromatography and preparative TLC and HPLC consecutively. The structures of the two compounds were elucidated by using high-performance liquid chromatography, using double online detection with a diode array spectrophotometer and a mass spectrometer (HPLC-DAD-ESI-MS), and extensive one- and two-dimensional NMR experiments. Both substances—1-methyl-β-carboline (harmane)(1) and 1-acetyl-4-methyl-β-carboline (2), are natural products and are isolated for the first time from the culture filtrate of the antarctic strain Thermoactinomyces sp. imbas-14. These compounds showed activity against important Gram-positive (Bacillus mycoides, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli), yeasts (Candida tropicalis) and filamentous fungi (Penicillium notatum).