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ABSTRACT
In order to elucidate the function of ATPase subuinit C from Antarctic notothenioid fishes, we designed and adopted a modified pEBV-VIGS (pea early browning virus, pEBV; virus-induced gene silencing, VIGS) virus infection technique (10) to introduce the ATPase gene into tobacco plants. The result of RT-PCR confirmed that the ATPase subuinit C gene was over-expressed in tobacco. In contrast to wild-type plants, these transgenic lines grew faster under low temperature. The physiological and biochemical effects of high-level expression of the pCAPE2-ATP in tobacco were then analyzed; the changes in electrolyte leakage activity, MDA content, proline content, soluble protein content and the ratio of Fv/Fm showed that ATP over-expression in tobacco increased the cold tolerance of plants. These results demonstrated that ATP can possibly be used to enhance the low temperature-tolerance of plants.