Chromosome Deletion and Translocation in Chinese Spring-Lophopyrum Elongatum 6E Disomic Addition Lines Induced by the Gametocidal Chromosome 2C from Aegilops Cylindrica

ABSTRACT

To produce wheat deletion lines and translocation lines for cytogenetic studies or breeding, a cross was made between the “Chinese Spring”-gametocidal chromosome 2C disomic addition line (CS-2C”) and the “Chinese Spring”-Lophopyrum elongatum 6E disomic addition line (CS-6E”). Meiosis of F₁ pollen mother cells (PMCs) was analyzed. F₂ plants derived from self-crossing of F hybrids were subjected to chromosome C-banding and genomic in situ hybridization (GISH) to detect chromosome variations. The results showed that large quantities of abnormal chromosomes in F₁PMCs were observed and 9 translocation plants and 10 wheat deletion plants were characterized among 95 progeny plants of F₂. There were seven wheat-L. elongatum translocation plants and two wheat intergenomic translocations in nine translocation plants. The translocation frequency was 7.37% and the deletion frequency was 10.5%. These results indicate that the induction of chromosomal variations using gametocidal chromosome 2C was efficient. Translocations with small alien segments and deletions with minimal loss of wheat chromatin were preferable for breeding purposes.

Keywords:

• gametocidal chromosome
• translocation
• deletion
• c-banding
• genomic in situ hybridization