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Emerging Microbes & Infections Volume 7, 2018 - Issue 1
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Original Articles

Sensitive detection of Treponema pallidum DNA from the whole blood of patients with syphilis by the nested PCR assay

Cuini WangSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
,
Yuanyuan ChengSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
,
Biao LiuSchool of Laboratory Medicine, Wenzhou Medical UniversityWenzhouChina;Clinical Laboratory CenterHunan Children’s HospitalChangshaChina
,
Yuanyuan WangShanghai Skin Disease Hospital, Clinical School of Anhui Medical UniversityShanghaiChina
,
Weiming GongSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
,
Yihong QianSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
,
Zhifang GuanSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
,
Haikong LuSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
,
Xin GuSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
,
Mei ShiSTD Institute, Shanghai Skin Disease HospitalShanghaiChina
&
Pingyu ZhouSTD Institute, Shanghai Skin Disease HospitalShanghaiChina;Shanghai Skin Disease Hospital, Clinical School of Anhui Medical UniversityShanghaiChinaCorrespondence[email protected]
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Pages 1-7 | Received 27 Dec 2017, Accepted 03 Apr 2018, Published online: 09 May 2018

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Xiaodong Wang, Paride Abliz & Shuwen Deng. (2024) Molecular Characteristics of Macrolide Resistance in Treponema pallidum from Patients with Latent Syphilis in Xinjiang, China. Infection and Drug Resistance 16, pages 1231-1236.
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Fan Gao, Pei Liu, Yaqian Huo, Lianlian Bian, Xing Wu, Mingchen Liu, Qian Wang, Qian He, Fangyu Dong, Zejun Wang, Zhongping Xie, Zhongyang Zhang, Meirong Gu, Yingzhi Xu, Yajing Li, Rui Zhu, Tong Cheng, Tao Wang, Qunying Mao & Zhenglun Liang. (2024) A screening study on the detection strain of Coxsackievirus A6: the key to evaluating neutralizing antibodies in vaccines. Emerging Microbes & Infections 13:1.
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Weiping Cao, Phoebe G. Thorpe, Kevin O’Callaghan & Ellen N. Kersh. (2023) Advantages and limitations of current diagnostic laboratory approaches in syphilis and congenital syphilis. Expert Review of Anti-infective Therapy 21:12, pages 1339-1354.
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Xiaoxia Duan, Chaoyong Zhang, Xiao Wang, Xueling Ren, Hongxia Peng, Xueqin Tang, Liangzhi Zhang, Zhenhua Chen, Yan Ye, Mengmou Zheng, Wanzhen Zhong, Xiyue Chen, Yilan Zeng, Ping Yuan & Lu Long. (2022) Molecular epidemiology and clinical features of hand, foot and mouth disease requiring hospitalization after the use of enterovirus A71 inactivated vaccine in chengdu, China, 2017-2022: a descriptive study. Emerging Microbes & Infections 11:1, pages 2510-2519.
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Muhammad Fazal Hameed, Yanan Chen, Ying Wang, Muhammad Shafiq, Hazrat Bilal, Linqing Liu, Jinming Ma, Pengying Gu & Honghua Ge. (2021) Epidemiological Characterization of Colistin and Carbapenem Resistant Enterobacteriaceae in a Tertiary: A Hospital from Anhui Province. Infection and Drug Resistance 14, pages 1325-1333.
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Articles from other publishers (19)

John R. Papp, Ina U. Park, Yetunde Fakile, Lara Pereira, Allan Pillay & Gail A. Bolan. (2024) CDC Laboratory Recommendations for Syphilis Testing, United States, 2024. MMWR. Recommendations and Reports 73:1, pages 1-32.
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Jacky Flipse, Anne-Marie Niekamp, Anne Dirks, Nicole H. T. M. Dukers-Muijrers, Christian J. P. A. Hoebe, Petra Wolffs & Inge H. M. van Loo. (2023) Refining Timely Diagnosis of Early Syphilis by Using Treponema pallidum PCR or IgM Immunoblotting Next to Conventional Serology for Syphilis. Journal of Clinical Microbiology 61:6.
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Miaomiao Li, Yan Lv, Dawei Cui, Yushan Xu, Mengjiao Lin, Xiaojun Zhang, Yongjun Wang, Cuifen Shen & Jue Xie. (2023) Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekeeping gene. BMC Infectious Diseases 23:1.
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Shahrukh Chaudhry, Idris Akinlusi, Ted Shi & Jorge Cervantes. (2023) Secondary Syphilis: Pathophysiology, Clinical Manifestations, and Diagnostic Testing. Venereology 2:2, pages 65-75.
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Katrin Hahn & Matthias Maschke. (2023) Neurolues. Krankenhaushygiene up2date 18:01, pages 57-70.
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Sukhum Silpa-archa, Tararat Hoopholerb & Charles Stephen Foster. (2022) Appraisal of vitreous syphilis antibody as a novel biomarker for the diagnosis of syphilitic uveitis: a prospective case-control study. Eye 37:1, pages 146-154.
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S A Nieuwenburg, H C A Zondag, S M Bruisten, V W Jongen, M F Schim van der Loeff, A P van Dam & H J C de Vries. (2022) Detection of Treponema pallidum DNA During Early Syphilis Stages in Peripheral Blood, Oropharynx, Ano-Rectum and Urine as a Proxy for Transmissibility . Clinical Infectious Diseases 75:6, pages 1054-1062.
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Katrin Hahn & Matthias Maschke. (2022) Neurolues. Neurologie up2date 05:02, pages 117-130.
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Krishnamoorthy Priya, Pasupathi Rathinasabapathi, Rex Arunraj, Dhanasekaran Sugapriya & Mohandass Ramya. (2022) Development of multiplex HRM-based loop-mediated isothermal amplification method for specific and sensitive detection of Treponema pallidum. Archives of Microbiology 204:6.
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Cuini Wang, Xin Zheng, Zhifang Guan, Danyang Zou, Xin Gu, Haikong Lu, Mei Shi & Pingyu Zhou. (2022) Quantified Detection of Treponema pallidum DNA by PCR Assays in Urine and Plasma of Syphilis Patients. Microbiology Spectrum 10:2.
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Júlio Henrique Ferreira de Sá Queiroz, Maisa Estopa Correa, Tiago da Silva Ferreira, Michele Ferreira Marques, Marcelo dos Santos Barbosa, Silvana Beutinger Marchioro & Simone Simionatto. (2022) Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction. Revista do Instituto de Medicina Tropical de São Paulo 64.
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Cuini Wang, Zhixiang Hu, Xin Zheng, Meiping Ye, Chunjie Liao, Mengya Shang, Weiming Gong, Zhifang Guan, Haikong Lu, Xin Gu, Mei Shi & Pingyu Zhou. (2021) A New Specimen for Syphilis Diagnosis: Evidence by High Loads of Treponema pallidum DNA in Saliva . Clinical Infectious Diseases 73:9, pages e3250-e3258.
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Damon Getman, Mike Lin, Nesreen Barakat, Rhonda Skvoretz, Charmie Godornes, Paul Swenson, Ashley Nenninger, Matthew R. Golden & Sheila A. Lukehart. (2021) Analytical Performance Characteristics of a New Transcription-Mediated Amplification Assay for Treponema pallidum. Journal of Clinical Microbiology 59:8.
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Alexandra K. Medoro & Pablo J. Sánchez. (2021) Syphilis in Neonates and Infants. Clinics in Perinatology 48:2, pages 293-309.
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Yuting Luo, Yafeng Xie & Yongjian Xiao. (2021) Laboratory Diagnostic Tools for Syphilis: Current Status and Future Prospects. Frontiers in Cellular and Infection Microbiology 10.
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N.N. Potekaev, N.V. Frigo, G.A. Dmitriev, N.V. Kitaeva, O.V. Dolya, A.E. Gushchin, E.S. Negasheva & S.G. Mardanli. (2021) Laboratory diagnostics of syphilis. Modern approaches and perspectives. Part I. Epidemiology. Direct diagnostic methods. Klinicheskaya dermatologiya i venerologiya 20:6, pages 7.
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Eliška Vrbová, Lenka Mikalová, Linda Grillová, Petra Pospíšilová, Radim Strnadel, Eliška Dastychová, Martina Kojanová, Miluše Kreidlová, Daniela Vaňousová, Filip Rob, Přemysl Procházka, Alena Krchňáková, Vladimír Vašků, Vladana Woznicová, Monika Dvořáková Heroldová, Ivana Kuklová, Hana Zákoucká & David Šmajs. (2020) A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results. PLOS ONE 15:8, pages e0237949.
Crossref
Man Xu, Yafeng Xie, Manyi Tan, Kang Zheng, Yongjian Xiao, Chuanhao Jiang, Feijun Zhao, Tiebing Zeng & Yimou Wu. (2019) The N‐terminal D1 domain of Treponema pallidum flagellin binding to TLR5 is required but not sufficient in activation of TLR5 . Journal of Cellular and Molecular Medicine 23:11, pages 7490-7504.
Crossref
Matthew Golden, Meghan O’Donnell, Sheila Lukehart, Paul Swenson, Paul Hovey, Charmie Godornes, Sarah Romano & Damon Getman. (2019) Treponema pallidum Nucleic Acid Amplification Testing To Augment Syphilis Screening among Men Who Have Sex with Men . Journal of Clinical Microbiology 57:8.
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