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Current Eye Research Volume 20, 2000 - Issue 5
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Research Article

Optimization of non-viral gene transfer to human primary retinal pigment epithelial cells

Khaled Abul-Hassan University Department of Ophthalmology, Manchester Royal Eye Hospital, Oxford Road, Manchester, UK; Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester, UK; Cell and Molecular Biology Unit, Department of Optometry and Vision Sciences, Cardiff University, Cardiff, UK
,
Richard Walmsley University Department of Ophthalmology, Manchester Royal Eye Hospital, Oxford Road, Manchester, UK; Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester, UK; Cell and Molecular Biology Unit, Department of Optometry and Vision Sciences, Cardiff University, Cardiff, UK
&
Mike Boulton University Department of Ophthalmology, Manchester Royal Eye Hospital, Oxford Road, Manchester, UK; Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester, UK; Cell and Molecular Biology Unit, Department of Optometry and Vision Sciences, Cardiff University, Cardiff, UK
Pages 361-366 | Published online: 02 Jul 2009

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Gabriele Thumann. (2011) Nonviral gene therapy for age-related macular degeneration. Expert Review of Ophthalmology 6:1, pages 81-93.
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Alexandru T Balaban & Marc Antoniu Ilies. (2001) Recent developments in cationic lipid-mediated gene delivery and gene therapy. Expert Opinion on Therapeutic Patents 11:11, pages 1729-1752.
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Articles from other publishers (23)

Bibhudatta Mishra, David R. Wilson, Srinivas R. Sripathi, Mark P. Suprenant, Yuan Rui, Karl J. Wahlin, Cynthia A. Berlinicke, Jordan J. Green & Donald J. Zack. (2019) A Combinatorial Library of Biodegradable Polyesters Enables Non-viral Gene Delivery to Post-Mitotic Human Stem Cell-Derived Polarized RPE Monolayers. Regenerative Engineering and Translational Medicine 6:3, pages 273-285.
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Ana del Pozo-Rodríguez, Josune Torrecilla, Alicia Rodríguez-Gascón & María Ángeles Solinís. 2018. Drug Delivery for the Retina and Posterior Segment Disease. Drug Delivery for the Retina and Posterior Segment Disease 131 149 .
Nooshin Tasharrofi, Fatemeh Kouhkan, Masoud Soleimani, Zahra-Soheila Soheili, Fatemeh Atyabi, Hamid Akbari Javar & Farid Abedin Dorkoosh. (2017) Efficient gene delivery to primary human retinal pigment epithelial cells: The innate and acquired properties of vectors. International Journal of Pharmaceutics 518:1-2, pages 66-79.
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M. Ángeles Solinís, Ana del Pozo-Rodríguez, Paola S. Apaolaza & Alicia Rodríguez-Gascón. (2015) Treatment of ocular disorders by gene therapy. European Journal of Pharmaceutics and Biopharmaceutics 95, pages 331-342.
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Alicia Rodríguez-Gascón, Ana del Pozo-Rodríguez, Arantxazu Isla & María Angeles Solinís. (2015) Vaginal gene therapy. Advanced Drug Delivery Reviews 92, pages 71-83.
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HONGLI LI, CAIFENG WAN & FENGHUA LI. (2015) Recombinant adeno-associated virus-, polyethylenimine/plasmid- and lipofectamine/carboxyfluorescein-labeled small interfering RNA-based transfection in retinal pigment epithelial cells with ultrasound and/or SonoVue. Molecular Medicine Reports 11:5, pages 3609-3614.
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Panagiotis Mastorakos, Siva P. Kambhampati, Manoj K. Mishra, Tony Wu, Eric Song, Justin Hanes & Rangaramanujam M. Kannan. (2015) Hydroxyl PAMAM dendrimer-based gene vectors for transgene delivery to human retinal pigment epithelial cells. Nanoscale 7:9, pages 3845-3856.
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R. Herrero-Vanrell, M. Vicario de la Torre, V. Andrés-Guerrero, D. Barbosa-Alfaro, I.T. Molina-Martínez & I. Bravo-Osuna. (2013) Nano and microtechnologies for ophthalmic administration, an overview. Journal of Drug Delivery Science and Technology 23:2, pages 75-102.
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Joel C. Sunshine, Sarah B. Sunshine, Imran Bhutto, James T. Handa & Jordan J. Green. (2012) Poly(?-Amino Ester)-Nanoparticle Mediated Transfection of Retinal Pigment Epithelial Cells In Vitro and In Vivo. PLoS ONE 7:5, pages e37543.
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Diego DelgadoAna del Pozo-RodríguezMaria Ángeles SolinísMarcelino Avilés-TriquerosBernhard H.F. WeberEduardo FernándezAlicia R. Gascón. (2012) Dextran and Protamine-Based Solid Lipid Nanoparticles as Potential Vectors for the Treatment of X-Linked Juvenile Retinoschisis. Human Gene Therapy 23:4, pages 345-355.
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Jing Du, Qiu Sheng Shi, Ying Sun, Pei Feng Liu, Ming Jie Zhu, Lian Fang Du & You Rong Duan. (2011) Enhanced delivery of monomethoxypoly(ethylene glycol)‐poly(lactic‐co‐glycolic acid)‐poly l ‐lysine nanoparticles loading platelet‐derived growth factor BB small interfering RNA by ultrasound and/or microbubbles to rat retinal pigment epithelium cells . The Journal of Gene Medicine 13:6, pages 312-323.
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Tamás Juhász, Csaba Matta, Zoltán Mészár, Georgina Nagy, Zsolt Szíjgyártó, Zsanett Molnár, Bernadett Kolozsvári, Éva Bakó & Róza Zákány. (2010) Optimalized transient transfection of chondrogenic primary cell cultures. Open Life Sciences 5:5, pages 572-584.
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Rangeetha Naik, Arijit Mukhopadhyay & Munia Ganguli. (2009) Gene delivery to the retina: focus on non-viral approaches. Drug Discovery Today 14:5-6, pages 306-315.
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L. Peeters, N.N. Sanders, A. Jones, J. Demeester & S.C. De Smedt. (2007) Post-pegylated lipoplexes are promising vehicles for gene delivery in RPE cells. Journal of Controlled Release 121:3, pages 208-217.
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Elias Fattal & Amélie Bochot. (2006) Ocular delivery of nucleic acids: antisense oligonucleotides, aptamers and siRNA. Advanced Drug Delivery Reviews 58:11, pages 1203-1223.
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Margit Hornof, Elisa Toropainen & Arto Urtti. (2005) Cell culture models of the ocular barriers. European Journal of Pharmaceutics and Biopharmaceutics 60:2, pages 207-225.
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Haike Dannowski, Jürgen Bednarz, Regina Reszka, Katrin Engelmann & Uwe Pleyer. (2005) Lipid-mediated gene transfer of acidic fibroblast growth factor into human corneal endothelial cells. Experimental Eye Research 80:1, pages 93-101.
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Leena Pitkänen, Jukka Pelkonen, Marika Ruponen, Seppo Rönkkö & Arto Urtti. (2004) Neural retina limits the nonviral gene transfer to retinal pigment epithelium in an in vitro bovine eye model. The AAPS Journal 6:3, pages 72-80.
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Shigeru Kawakami, Ayaka Harada, Koji Sakanaka, Koyo Nishida, Junzo Nakamura, Toshiyuki Sakaeda, Nobuhiro Ichikawa, Mikiro Nakashima & Hitoshi Sasaki. (2004) In vivo gene transfection via intravitreal injection of cationic liposome/plasmid DNA complexes in rabbits. International Journal of Pharmaceutics 278:2, pages 255-262.
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Mika Reinisalo, Arto Urtti & Paavo Honkakoski. (2003) Retina-specific gene expression and improved DNA transfection in WERI-Rb1 retinoblastoma cells. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1628:3, pages 169-176.
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Ingrid Hilger, Sylvia Frühauf, Werner Linß, Robert Hiergeist, Wilfried Andrä, Rudolf Hergt & Werner A Kaiser. (2003) Cytotoxicity of selected magnetic fluids on human adenocarcinoma cells. Journal of Magnetism and Magnetic Materials 261:1-2, pages 7-12.
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Soma De & Thomas P. Sakmar. (2002) Interaction of A2E with Model Membranes. Implications to the Pathogenesis of Age-related Macular Degeneration. The Journal of General Physiology 120:2, pages 147-157.
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C.K. Kim, Kh.H. Haider, S.H. Choi, E.J. Choi, W.S. Ahn & Y.B. Kim. (2002) Nonviral Vector for Efficient Gene Transfer to Human Ovarian Adenocarcinoma Cells. Gynecologic Oncology 84:1, pages 85-93.
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