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New Zealand Journal of Botany Volume 31, 1993 - Issue 4
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Physiology and biochemistry

Preparation and preservation of nuclei from plant tissues for quantitative DNA analysis by flow cytometry

M. E. Hopping Mt Albert Research Centre, The Horticulture and Food Research Institute of New Zealand Ltd, Private Bag 96 169, Auckland, New Zealand; Levin Research Centre, Private Bag 4005, Levin, New Zealand
Pages 391-401 | Received 18 Feb 1993, Accepted 16 Jul 1993, Published online: 19 Dec 2011

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G. C. Lindsay. (1995) DNA content of nuclei of Actinidia deliciosa var. deliciosa ‘Hayward’ (kiwifruit) analysed by image cytometry. New Zealand Journal of Botany 33:2, pages 243-255.
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G. C. Lindsay, M. E. Hopping, H. Binding & G. K. Burge. (1995) Graft chimeras and somatic hybrids for new cultivars. New Zealand Journal of Botany 33:1, pages 79-92.
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M. E. Hopping. (1994) Seasonal changes in fluorescence intensity of kiwifruit nuclei stained with propidium iodide and measured by flow cytometry. New Zealand Journal of Botany 32:2, pages 237-245.
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M. E. Hopping. (1994) Flow cytometric analysis of Actinidia species. New Zealand Journal of Botany 32:1, pages 85-93.
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Articles from other publishers (26)

Maria Fomicheva & Elena Domblides. (2023) Mastering DNA Content Estimation by Flow Cytometry as an Efficient Tool for Plant Breeding and Biodiversity Research. Methods and Protocols 6:1, pages 18.
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João Loureiro, Martin Čertner, Magdalena Lučanová, Elwira Sliwinska, Filip Kolář, Jaroslav Doležel, Sònia Garcia, Sílvia Castro & David W. Galbraith. 2023. Plant Cytogenetics and Cytogenomics. Plant Cytogenetics and Cytogenomics 25 64 .
Martin Čertner, Magdalena Lučanová, Elwira Sliwinska, Filip Kolář & João Loureiro. (2021) Plant material selection, collection, preservation, and storage for nuclear DNA content estimation . Cytometry Part A 101:9, pages 737-748.
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Saravanakumar Somasundaram, Viswanathan Satheesh, Major Singh & Sivalingam Anandhan. (2021) A simple flow cytometry-based assay to study global methylation levels in onion, a non-model species. Physiology and Molecular Biology of Plants 27:8, pages 1859-1865.
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Sergio J. Ochatt & Jose M. Seguí-Simarro. 2021. Doubled Haploid Technology. Doubled Haploid Technology 105 125 .
Shan Yang, Kai Zeng, Ling Luo, Wang Qian, Zhiqiang Wang, Jaroslav Doležel, Muqing Zhang, Xiangxiong Gao & Zuhu Deng. (2020) A flow cytometry-based analysis to establish a cell cycle synchronization protocol for Saccharum spp.. Scientific Reports 10:1.
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Sigita Bogačiovienė, Laima Česonienė, Sezai Ercisli, Andrius Valatavičius, Baltramiejus Jakštys, Saulius Šatkauskas & Algimantas Paulauskas. (2019) Ploidy levels and genetic diversity of Actinidia arguta (Siebold & Zucc.) Planch. ex Miq., A. kolomikta (Rupr. & Maxim.) Maxim., A. callosa Lindl., and A. melanandra Franch., accessions. Genetic Resources and Crop Evolution 66:5, pages 1107-1118.
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. 2016. Kiwifruit. Kiwifruit 309 328 .
Hongwen HUANG. 2016. Kiwifruit. Kiwifruit 9 44 .
Allan Ross Ferguson & Hongwen Huang. 2016. The Kiwifruit Genome. The Kiwifruit Genome 55 63 .
Jan Vrána, Petr Cápal, Martina Bednářová & Jaroslav Doležel. 2014. Applied Plant Cell Biology. Applied Plant Cell Biology 395 430 .
Filip Kolář, Magdalena Lučanová, Jakub Těšitel, João Loureiro & Jan Suda. (2012) Glycerol-treated nuclear suspensions—an efficient preservation method for flow cytometric analysis of plant samples. Chromosome Research 20:2, pages 303-315.
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S. J. Ochatt, E. M. Patat-Ochatt & A. Moessner. (2011) Ploidy level determination within the context of in vitro breeding. Plant Cell, Tissue and Organ Culture (PCTOC) 104:3, pages 329-341.
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P. M. Datson & A. R. Ferguson. 2011. Wild Crop Relatives: Genomic and Breeding Resources. Wild Crop Relatives: Genomic and Breeding Resources 1 20 .
Eduardo Cires, Candela Cuesta, Elena L. Peredo, María Ángeles Revilla & José Antonio Fernández Prieto. (2009) Genome size variation and morphological differentiation within Ranunculus parnassifolius group (Ranunculaceae) from calcareous screes in the Northwest of Spain. Plant Systematics and Evolution 281:1-4, pages 193-208.
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Harshi K. Gamage & Susanne Schmidt. (2009) Short Communication. A robust method for chromosome quantification and ploidy determination in woody species. Australian Journal of Botany 57:2, pages 87.
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Sergio J. Ochatt. (2008) Flow cytometry in plant breeding. Cytometry Part A 73A:7, pages 581-598.
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Jan Suda, Paul Kron, Brian C. Husband & Pavel Trávníček. 2007. Flow Cytometry with Plant Cells. Flow Cytometry with Plant Cells 103 130 .
Jan Suda & Pavel Trávníček. (2006) Estimation of Relative Nuclear DNA Content in Dehydrated Plant Tissues by Flow Cytometry. Current Protocols in Cytometry 38:1.
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Jan Suda & Pavel Trávníček. (2006) Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry—new prospects for plant research. Cytometry Part A 69A:4, pages 273-280.
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P. Šmarda & D. Stančík. (2006) Ploidy Level Variability in South American Fescues (Festuca L., Poaceae): Use of Flow Cytometry in Up to 5 1 / 2 -Year-Old Caryopses and Herbarium Specimens . Plant Biology 8:1, pages 73-80.
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C. Kevers, R. Greimers, T. Franck, B. Bisbis, J. Dommes & T. Gaspar. (1999) Flow Cytometry Estimation of Nuclear Size and Ploidy Level of Habituated Calli of Sugar Beet. Biologia plantarum 42:3, pages 321-332.
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Wandee Yanpaisan, Nicholas J.C. King & Pauline M. Doran. (1999) Flow cytometry of plant cells with applications in large-scale bioprocessing. Biotechnology Advances 17:1, pages 3-27.
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David H. Lewis, Garry K. Burge, Murray E. Hopping & Paula E. Jameson. (2006) Cytokinins and fruit development in the kiwifruit ( Actinidia deliciosa ). II. Effects of reduced pollination and CPPU application . Physiologia Plantarum 98:1, pages 187-195.
Crossref
E. R. Morgan, G. K. Burge, J. F. Seelye, J. E. Grant & M. E. Hopping. (1995) Interspecific hybridisation betweenLimonium perigrinum Bergius andLimonium purpuratum L.. Euphytica 83:3, pages 215-224.
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G. C. Lindsay, M. E. Hopping & I. E. W. O'Brien. (1994) Detection of protoplast-derived DNA tetraploid Lisianthus (Eustoma grandiflorum) plants by leaf and flower characteristics and by flow cytometry. Plant Cell, Tissue and Organ Culture 38:1, pages 53-55.
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