322
Views
39
CrossRef citations to date
0
Altmetric
ORIGINAL ARTICLE

Improvement of real‐time polymerase chain reaction for quantifying TNF‐α mRNA expression in inflamed colorectal mucosa: An approach to optimize procedures for clinical use

, , , , &
Pages 249-259 | Received 19 Aug 2005, Accepted 11 Jan 2006, Published online: 08 Jul 2009

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (8)

Jon Florholmen. (2015) Mucosal healing in the era of biologic agents in treatment of inflammatory bowel disease. Scandinavian Journal of Gastroenterology 50:1, pages 43-52.
Read now
Renathe Rismo, Trine Olsen, Guanglin Cui, Eyvind J. Paulssen, Ingrid Christiansen, Knut Johnsen, Jon Florholmen & Rasmus Goll. (2013) Normalization of mucosal cytokine gene expression levels predicts long-term remission after discontinuation of anti-TNF therapy in Crohn's disease. Scandinavian Journal of Gastroenterology 48:3, pages 311-319.
Read now
Renathe Rismo, Trine Olsen, Guanglin Ciu, Eyvind J. Paulssen, Ingrid Christiansen, Jon Florholmen & Rasmus Goll. (2012) The effect of adalimumab for induction of endoscopic healing and normalization of mucosal cytokine gene expression in Crohn's disease. Scandinavian Journal of Gastroenterology 47:10, pages 1200-1210.
Read now
Renathe Rismo, Trine Olsen, Guanglin Cui, Ingrid Christiansen, Jon Florholmen & Rasmus Goll. (2012) Mucosal cytokine gene expression profiles as biomarkers of response to infliximab in ulcerative colitis. Scandinavian Journal of Gastroenterology 47:5, pages 538-547.
Read now
Jon Florholmen & Walter Fries. (2011) Candidate mucosal and surrogate biomarkers of inflammatory bowel disease in the era of new technology. Scandinavian Journal of Gastroenterology 46:12, pages 1407-1417.
Read now
Trine Olsen, Guanglin Cui, Rasmus Goll, Anne Husebekk & Jon Florholmen. (2009) Infliximab therapy decreases the levels of TNF-α and IFN-γ mRNA in colonic mucosa of ulcerative colitis. Scandinavian Journal of Gastroenterology 44:6, pages 727-735.
Read now
Dominic T. K. Keating, Andrea P. Malizia, D. Sadlier, C. Hurson, A. E. Wood, J. McCarthy, L. Nolke, Jim J. Egan & Peter P. Doran. (2008) LUNG TISSUE STORAGE: OPTIMIZING CONDITIONS FOR FUTURE USE IN MOLECULAR RESEARCH. Experimental Lung Research 34:8, pages 455-466.
Read now
Trine Olsen, Rasmus Goll, Guanglin Cui, Anne Husebekk, Barthold Vonen, Grethe støa Birketvedt & Jon Florholmen. (2007) Tissue levels of tumor necrosis factor-alpha correlates with grade of inflammation in untreated ulcerative colitis. Scandinavian Journal of Gastroenterology 42:11, pages 1312-1320.
Read now

Articles from other publishers (31)

Guanglin Cui, Aping Yuan, Zhigang Pang & Jon Florholmen. (2023) Differential profile of protumor immunological factors between the tumor site and the tumor-free site – predictive potential of IL-8 and COX2 for colorectal cancer and metastasis. International Immunopharmacology 118, pages 110089.
Crossref
Hyo-Joon Yang, Seung In Seo, Jin Lee, Cheal Wung Huh, Joon Sung Kim, Jun Chul Park, Hyunki Kim, Hakdong Shin, Cheol Min Shin, Chan Hyuk Park & Sang Kil Lee. (2023) Sample Collection Methods in Upper Gastrointestinal Research. Journal of Korean Medical Science 38:32.
Crossref
Rasmus Goll, Øystein K. Moe, Kay-Martin Johnsen, Renate Meyer, Joachim Friestad, Mona D. Gundersen, Hege Kileng, Knut Johnsen & Jon R. Florholmen. (2022) Pharmacodynamic mechanisms behind a refractory state in inflammatory bowel disease. BMC Gastroenterology 22:1.
Crossref
Kay-Martin Johnsen, Jon Florholmen, Øystein K. Moe, Mona Gundersen, Julia Beilfuss, Hege Kileng, Sveinung W. Sørbye & Rasmus Goll. (2022) Prediction of long-term remission in patients following discontinuation of anti-TNF therapy in ulcerative colitis: a 10 year follow up study. BMC Gastroenterology 22:1.
Crossref
Guanglin Cui, Gui Li, Zhigang Pang, Jon Florholmen & Rasmus Goll. (2022) The presentation and regulation of the IL-8 network in the epithelial cancer stem-like cell niche in patients with colorectal cancer. Biomedicine & Pharmacotherapy 152, pages 113252.
Crossref
Guanglin Cui, Jon Florholmen & Rasmus Goll. (2022) Could Mucosal TNF Transcript as a Biomarker Candidate Help Optimize Anti-TNF Biological Therapy in Patients With Ulcerative Colitis?. Frontiers in Immunology 13.
Crossref
Guanglin Cui, Zhenfeng Li, Jon Florholmen & Rasmus Goll. (2021) Dynamic stromal cellular reaction throughout human colorectal adenoma-carcinoma sequence: A role of TH17/IL-17A. Biomedicine & Pharmacotherapy 140, pages 111761.
Crossref
Ewa Żyła, Katarzyna Dziendzikowska, Dariusz Kamola, Jacek Wilczak, Rafał Sapierzyński, Joanna Harasym & Joanna Gromadzka-Ostrowska. (2021) Anti-Inflammatory Activity of Oat Beta-Glucans in a Crohn’s Disease Model: Time- and Molar Mass-Dependent Effects. International Journal of Molecular Sciences 22:9, pages 4485.
Crossref
Jon R. Florholmen, Kay-Martin Johnsen, Renate Meyer, Trine Olsen, Øystein K. Moe, Petter Tandberg, Mona D. Gundersen, Jan-Magnus Kvamme, Knut Johnsen, Terje Løitegård, Gabriele Raschpichler, Cecilia Vold, Sveinung W. Sørbye & Rasmus Goll. (2020) Discovery and validation of mucosal TNF expression combined with histological score - a biomarker for personalized treatment in ulcerative colitis. BMC Gastroenterology 20:1.
Crossref
Guanglin Cui, Aping Yuan, Zhenfeng Li, Rasmus Goll & Jon Florholmen. (2020) ST2 and regulatory T cells in the colorectal adenoma/carcinoma microenvironment: implications for diseases progression and prognosis. Scientific Reports 10:1.
Crossref
Guanglin Cui, Aping Yuan, Zhenglu Sun, Wei Zheng & Zhigang Pang. (2018) IL-1β/IL-6 network in the tumor microenvironment of human colorectal cancer. Pathology - Research and Practice 214:7, pages 986-992.
Crossref
Guanglin Cui, Aping Yuan, Li Zhu, Jon Florholmen & Rasmus Goll. (2017) Increased expression of interleukin-21 along colorectal adenoma-carcinoma sequence and its predicating significance in patients with sporadic colorectal cancer. Clinical Immunology 183, pages 266-272.
Crossref
Kay-Martin Johnsen, Rasmus Goll, Vegard Hansen, Trine Olsen, Renathe Rismo, Richard Heitmann, Mona D. Gundersen, Jan M. Kvamme, Eyvind J. Paulssen, Hege Kileng, Knut Johnsen & Jon Florholmen. (2017) Repeated intensified infliximab induction – results from an 11-year prospective study of ulcerative colitis using a novel treatment algorithm. European Journal of Gastroenterology & Hepatology 29:1, pages 98-104.
Crossref
Mona Dixon Gundersen, Rasmus Goll, Johanna Hol, Trine Olsen, Renathe Rismo, Sveinung W. Sørbye, Olav Sundnes, Guttorm Haraldsen & Jon Florholmen. (2016) Loss of interleukin 33 expression in colonic crypts - a potential marker for disease remission in ulcerative colitis. Scientific Reports 6:1.
Crossref
Trine Olsen, Renathe Rismo, Mona Dixon Gundersen, Eyvind J. Paulssen, Knut Johnsen, Jan-Magnus Kvamme, Rasmus Goll & Jon Florholmen. (2016) Normalization of mucosal tumor necrosis factor-α: A new criterion for discontinuing infliximab therapy in ulcerative colitis. Cytokine 79, pages 90-95.
Crossref
Wei Hua, Aping Yuan, Wei Zheng, Can Li, Jing Cui, Zhigang Pang, Li Zhang, Zhenfeng Li, Rasmus Goll & Guanglin Cui. (2016) Accumulation of FoxP3+ T regulatory cells in the tumor microenvironment of human colorectal adenomas. Pathology - Research and Practice 212:2, pages 106-112.
Crossref
Zana Sijan, Dagmara S. Antkiewicz, Jongbae Heo, Norman Y. Kado, James J. Schauer, Constantinos Sioutas & Martin M. Shafer. (2015) An In Vitro alveolar macrophage assay for the assessment of inflammatory cytokine expression induced by atmospheric particulate matter . Environmental Toxicology 30:7, pages 836-851.
Crossref
Guanglin Cui, Haili Qi, Mona D. Gundersen, Hang Yang, Ingrid Christiansen, Sveinung W. Sørbye, Rasmus Goll & Jon Florholmen. (2014) Dynamics of the IL-33/ST2 network in the progression of human colorectal adenoma to sporadic colorectal cancer. Cancer Immunology, Immunotherapy 64:2, pages 181-190.
Crossref
Ian McGowan, Laura Janocko, Shaun Burneisen, Anand Bhat & Nicola Richardson-Harman. (2015) Variability of cytokine gene expression in intestinal tissue and the impact of normalization with the use of reference genes. Cytokine 71:1, pages 81-88.
Crossref
Guanglin Cui, Hang Yang, Jianbo Zhao, Aping Yuan & Jon Florholmen. (2014) Elevated Proinflammatory Cytokine IL-17A in the Adjacent Tissues Along the Adenoma-Carcinoma Sequence. Pathology & Oncology Research 21:1, pages 139-146.
Crossref
Trine Olsen, Renathe Rismo, Guanglin Cui, Rasmus Goll, Ingrid Christiansen & Jon Florholmen. (2011) TH1 and TH17 interactions in untreated inflamed mucosa of inflammatory bowel disease, and their potential to mediate the inflammation. Cytokine 56:3, pages 633-640.
Crossref
Marco Daperno, Fabiana Castiglione, Lissy de Ridder, Iris Dotan, Martti Färkkilä, Jon Florholmen, Gerald Fraser, Walter Fries, Xavier Hebuterne, Peter Laszlo Lakatos, Julián Panés, Jordi Rimola & Edouard Louis. (2011) Results of the 2nd part Scientific Workshop of the ECCO (II): Measures and markers of prediction to achieve, detect, and monitor intestinal healing in Inflammatory Bowel Disease. Journal of Crohn's and Colitis 5:5, pages 484-498.
Crossref
Jon Sponheim, Jürgen Pollheimer, Trine Olsen, Johanna Balogh, Clara Hammarström, Tamara Loos, Monika Kasprzycka, Dag Reidar Sørensen, Hogne Røed Nilsen, Axel M. Küchler, Morten H. Vatn & Guttorm Haraldsen. (2010) Inflammatory Bowel Disease-Associated Interleukin-33 Is Preferentially Expressed in Ulceration-Associated Myofibroblasts. The American Journal of Pathology 177:6, pages 2804-2815.
Crossref
Sara Bogaert, Debby Laukens, Harald Peeters, Lode Melis, Kim Olievier, Nico Boon, Gust Verbruggen, Jo Vandesompele, Dirk Elewaut & Martine De Vos. (2010) Differential mucosal expression of Th17-related genes between the inflamed colon and ileum of patients with inflammatory bowel disease. BMC Immunology 11:1.
Crossref
Daniel J. B. Marks, Farooq Z. Rahman, Gavin W. Sewell & Anthony W. Segal. (2009) Crohn’s Disease: an Immune Deficiency State. Clinical Reviews in Allergy & Immunology 38:1, pages 20-31.
Crossref
Guanglin Cui, Aping Yuan, Rasmus Goll, Barthold Vonen & Jon Florholmen. (2009) Dynamic changes of interleukin-8 network along the colorectal adenoma–carcinoma sequence. Cancer Immunology, Immunotherapy 58:11, pages 1897-1905.
Crossref
APING YUAN, SONJA E. STEIGEN, RASMUS GOLL, BARTHOLD VONEN, ANNE HUSBEKK, GUANGLIN CUI & JON FLORHOLMEN. (2008) Dendritic cell infiltration pattern along the colorectal adenoma-carcinoma sequence. APMIS 116:6, pages 445-456.
Crossref
R. Goll, G. Cui, T. Olsen, V. Isaksen, F. Gruber, A. Husebekk & J. Florholmen. (2007) Alterations in Antral Cytokine Gene Expression in Peptic Ulcer Patients During Ulcer Healing and After Helicobacter pylori Eradication. Scandinavian Journal of Immunology 0:0, pages 071120035119001-???.
Crossref
Guanglin Cui, Aping Yuan, Rasmus Goll, Trine Olsen, Anne Husebekk, Barthold Vonen & Jon Florholmen. (2007) Distinct changes of dendritic cell number and IL-12 mRNA level in adjacent mucosa throughout the colorectal adenoma–carcinoma sequence. Cancer Immunology, Immunotherapy 56:12, pages 1993-2001.
Crossref
Guanglin Cui, Rasmus Goll, Trine Olsen, Sonja Eriksson Steigen, Anne Husebekk, Barthold Vonen & Jon Florholmen. (2006) Reduced expression of microenvironmental Th1 cytokines accompanies adenomas–carcinomas sequence of colorectum. Cancer Immunology, Immunotherapy 56:7, pages 985-995.
Crossref
Rasmus Goll, Franz Gruber, Trine Olsen, Guanglin Cui, Gabriele Raschpichler, Magne Buset, Anne M. Asfeldt, Anne Husebekk & Jon Florholmen. (2007) Helicobacter pylori Stimulates a Mixed Adaptive Immune Response with a Strong T-Regulatory Component in Human Gastric Mucosa. Helicobacter 12:3, pages 185-192.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.