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Scandinavian Journal of Clinical and Laboratory Investigation Volume 67, 2007 - Issue 4
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Original Article

Is flow cytometry really adapted to the determination of sperm concentration?

J.‐C. Lu Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, P. R. China; Department of Laboratory Science, Nanjing Hospital, Jiangsu Corps, the Armed Police Force, PLA, Nanjing, P. R. China
,
F. Chen Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, P. R. China; Department of Microbiology and Immunology, Basic Medical College of Nanjing Medical University, Nanjing, P. R. China
,
H.‐R. Xu Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, P. R. China; Life and Science College, Nanjing Normal University, Nanjing, P. R. China
,
Y.‐M. Wu Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, P. R. China; Life and Science College, Nanjing Normal University, Nanjing, P. R. China
,
X.‐Y. Xia Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, P. R. China
,
Y.‐F. Huang Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, P. R. China
&
N.‐Q. Lu Jiangsu Family Planning Research Institute, Nanjing, P. R. China
 show all
Pages 394-401 | Received 15 Oct 2006, Accepted 13 Nov 2006, Published online: 08 Jul 2009

Citations (16)

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Read on this site (2)

Jin-Chun Lu, Yu-Feng Huang & Nian-Qing Lu. (2008) Antisperm immunity and infertility. Expert Review of Clinical Immunology 4:1, pages 113-126.
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T. B. Haugen. (2007) In search of an accurate and rapid method for sperm counting. Scandinavian Journal of Clinical and Laboratory Investigation 67:4, pages 436-438.
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Articles from other publishers (14)

Carminda Sandra Brito Salmito Vanderley, Marcelo José da Ascensão Feitosa Vieira, Thais Maia Torres, Marcos Luiz da Silva Apoliano, Carlos Henrique Sousa de Melo, Cristiane Clemente de Mello Salgueiro & José Ferreira Nunes. (2023) Determinação da concentração espermática de tambaqui (Colossoma macropomum) por espectrofotometria. Ciência Animal Brasileira 24.
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Carminda Sandra Brito Salmito Vanderley, Marcelo José da Ascensão Feitosa Vieira, Thais Maia Torres, Marcos Luiz da Silva Apoliano, Carlos Henrique Sousa de Melo, Cristiane Clemente de Mello Salgueiro & José Ferreira Nunes. (2023) Spectrophotometric method for determination of Colossoma macropomum (tambaqui) sperm concentration. Ciência Animal Brasileira 24.
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Adelina-Elisa Olbrich, Biwen A An Stepec, Nina Wurzler, Estela C Terol, Andrea Koerdt & Björn Meermann. (2022) Beyond corrosion: development of a single cell-ICP-ToF-MS method to uncover the process of microbiologically influenced corrosion. Metallomics 14:11.
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Scott Madill & Margaret V. Root Kustritz. 2020. Veterinary Cytology. Veterinary Cytology 531 551 .
Leonardo F.C. Brito, Gary C. Althouse, Christine Aurich, Peter J. Chenoweth, Bruce E. Eilts, Charles C. Love, Gaia C. Luvoni, Jere R. Mitchell, Augustine T. Peter, David G. Pugh & Dagmar Waberski. (2016) Andrology laboratory review: Evaluation of sperm concentration. Theriogenology 85:9, pages 1507-1527.
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Huiping Yang, Jonathan Daly & Terrence R. Tiersch. (2015) Determination of sperm concentration using flow cytometry with simultaneous analysis of sperm plasma membrane integrity in zebrafish Danio rerio . Cytometry Part A 89:4, pages 350-356.
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Anthony J.F. Reardon, Janet A.W. Elliott & Locksley E. McGann. (2014) Fluorescence as an alternative to light-scatter gating strategies to identify frozen–thawed cells with flow cytometry. Cryobiology 69:1, pages 91-99.
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AM Petrunkina & RAP Harrison. (2013) Fluorescence Technologies for Evaluating Male Gamete (Dys)Function. Reproduction in Domestic Animals 48:s1, pages 11-24.
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Jonathan Daly & Terrence R. Tiersch. (2012) Sources of variation in flow cytometric analysis of aquatic species sperm: The effect of cryoprotectants on flow cytometry scatter plots and subsequent population gating. Aquaculture 370-371, pages 179-188.
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Md. Sharoare Hossain, Anders Johannisson, Margareta Wallgren, Szabolcs Nagy, Amanda Pimenta Siqueira & Heriberto Rodriguez-Martinez. (2011) Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art. Asian Journal of Andrology 13:3, pages 406-419.
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A. M. Petrunkina & R. A. P. Harrison. (2011) Cytometric solutions in veterinary andrology: Developments, advantages, and limitations. Cytometry Part A 79A:5, pages 338-348.
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F Martínez‐Pastor, M Mata‐Campuzano, M Álvarez‐Rodríguez, M Álvarez, L Anel & P De Paz. (2010) Probes and Techniques for Sperm Evaluation by Flow Cytometry. Reproduction in Domestic Animals 45:s2, pages 67-78.
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A.M. Petrunkina & R.A.P. Harrison. (2010) Systematic misestimation of cell subpopulations by flow cytometry: A mathematical analysis. Theriogenology 73:7, pages 839-847.
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Jin-Chun Lu, Hong-Ye Zhang, Yu-An Hu, Yu-Feng Huang & Nian-Qing Lü. (2009) A survey on the status of semen analysis in 118 laboratories in China. Asian Journal of Andrology 12:1, pages 104-110.
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