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Scandinavian Journal of Clinical and Laboratory Investigation Volume 20, 1967 - Issue 1
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Original Article

Enzymatic Determination of Pyridoxal Phosphate in Plasma by Decarboxylation of L-Tyrosine-14 C (U) and a Comparison with the Tryptophan Load Test

A. Hamfelt Department of Clinical Chemistry, University Hospital, Uppsala, Sweden
Pages 1-10 | Received 11 Jan 1967, Published online: 28 Aug 2009

Citations (39)

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Read on this site (7)

Tim M Reynolds, Paul D Marshall & Andy M Brain. (1992) Hip fracture patients may be vitamin B6 deficient. Acta Orthopaedica Scandinavica 63:6, pages 635-638.
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Anders Hugoson & Björn Thorstensson. (1991) Vitamin B status and response to replacement therapy in patients with burning mouth syndrome. Acta Odontologica Scandinavica 49:6, pages 367-375.
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Arne Hamfelt. (1986) A Simplified Method for Determination of Pyridoxal Phosphate in Biological Samples. Upsala Journal of Medical Sciences 91:1, pages 105-109.
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Arne Hamfelt, Lars Söderhjelm & Gunlög Mikaelsson. (1984) Plasma Pyridoxal Phosphate in Women Taking Oral Contraceptives since at Least Five Years. Upsala Journal of Medical Sciences 89:3, pages 285-286.
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Alexander Botteri, Arne Hamfelt & Lars Söderhjelm. (1984) Pyridoxal Phosphate, Tryptophan, and Tyrosine in Blood and Cerebrospinal Fluid in Elderly Patients. Upsala Journal of Medical Sciences 89:3, pages 279-284.
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Hans Wolf. (1974) Studies on Tryptophan Metabolism in Man: The Effect of Hormones and Vitamin B6 on Urinary Excretion of Metabolites of the Kynurenine Pathway: Part 2. Scandinavian Journal of Clinical and Laboratory Investigation 33:sup136, pages 89-182.
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Howerde E. Sauberlich, Richard P. Dowdy & James H. Skala. (1973) Laboratory Tests for the Assessment of Nutritional Status. CRC Critical Reviews in Clinical Laboratory Sciences 4:3, pages 215-340.
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Articles from other publishers (32)

Anthony Maus, Adam Girtman, Jessa Kiesling, Jennifer Faber & Stefan K.G. Grebe. (2023) Overcoming the chromatographic challenges when performing LC-MS/MS measurements of pyridoxal-5′-phosphate. Journal of Chromatography B 1217, pages 123605.
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David Furth-Walker, David Leibman & Andrew Smolen. (1990) Relationship between Blood, Liver and Brain Pyridoxal Phosphate and Pyridoxamine Phosphate Concentrations in Mice. The Journal of Nutrition 120:11, pages 1338-1343.
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Henry M. MiddletonIIIIII. (1990) Intestinal hydrolysis of pyridoxal 5′-phosphatein vitro andin vivo in the rat. Digestive Diseases and Sciences 35:1, pages 113-120.
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P. K. S. Liu & G. A. Rose. 1988. Oxalate Metabolism in Relation to Urinary Stone. Oxalate Metabolism in Relation to Urinary Stone 177 192 .
Henry M. MiddletonIIIIII. (1986) Intestinal hydrolysis of pyridoxal 5′-phosphate in vitro and in vivo in the rat. Gastroenterology 91:2, pages 343-350.
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Margaret L. Fonda. (1986) Evaluations of tyrosine apodecarboxylase assays for pyridoxal phosphate. Analytical Biochemistry 155:1, pages 14-22.
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HM MiddletonIIIIII. (1986) Intestinal hydrolysis of pyridoxal 5′-phosphate in vitro and in vivo in the rat: effect of ethanol. The American Journal of Clinical Nutrition 43:3, pages 374-381.
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Bruno Lequeu, Jean-Claude Guilland & Jacques Klepping. (1985) Measurement of plasma pyridoxal 5′-phosphate by combination of an enzymatic assay with high-performance liquid chromatography/electrochemistry. Analytical Biochemistry 149:2, pages 296-300.
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A. M. Gressner & Daniela Sittel. (1985) Plasma Pyridoxal 5'-Phosphate Concentrations in Relation to Apo-Aminotransferase Levels in Normal, Uraemic, and Post-Myocardial Infarct Sera. Clinical Chemistry and Laboratory Medicine 23:10.
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Lawrence Lumeng, Alec Lui & Tjien O. Oei. (1984) Comparison of the Use of L-Tyrosine Apodecarboxylase and D-Serine Apodehydratase for Plasma Pyridoxal Phosphate Assay. The Journal of Nutrition 114:2, pages 385-392.
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H M MiddletonIIIIII, L R Mills & M Singh. (1984) Effect of ethanol on the uptake of pyridoxine HCl in the rat jejunum. The American Journal of Clinical Nutrition 39:1, pages 54-61.
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Henry M. MiddletonIIIIII. (1982) Characterization of Pyridoxal 5′-Phosphate Disappearance from In vivo Perfused Segments of Rat Jejunum. The Journal of Nutrition 112:2, pages 269-275.
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Bob Inyu Yang, Ashok K. Sawhney, Ronnie C. Pitchlyn & Peter M. Peer. 1981. Methods in Vitamin B-6 Nutrition. Methods in Vitamin B-6 Nutrition 79 98 .
Ting-Kai Li & Lawrence Lumeng. 1981. Methods in Vitamin B-6 Nutrition. Methods in Vitamin B-6 Nutrition 289 296 .
J. EJDERHAMN & A. HAMFELT. (1980) PYRIDOXAL PHOSPHATE CONCENTRATION IN BLOOD IN NEWBORN INFANTS AND THEIR MOTHERS COMPARED WITH THE AMOUNT OF EXTRA PYRIDOXOL TAKEN DURING PREGNANCY AND BREAST FEEDING. Acta Paediatrica 69:3, pages 327-330.
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Stephen T. Worland & Jules A. Shafer. (1980) A convenient lactic dehydrogenase-coupled assay for determining pyridoxal 5′-phosphate in plasma. Analytical Biochemistry 103:2, pages 323-330.
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Henry M. MiddletonIIIIII. (1979) Intestinal Absorption of Pyridoxal-5′-Phosphate: Disappearance from Perfused Segments of Rat Jejunum In Vivo. The Journal of Nutrition 109:6, pages 975-981.
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TH Parker, JP Marshall2nd2nd, RK Roberts, S Wang, ER Schiff, GR Wilkinson & S Schenker. (1979) Effect of acute alcohol ingestion on plasma pyridoxal 5′-phosphate. The American Journal of Clinical Nutrition 32:6, pages 1246-1252.
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Gul Ismahan & Simone Parvez. (1978) Effect of Starvation and Pattern of Feeding upon Activities of Enzymes Catechol-O-methyltransferase and Monoamine Oxidase in Heart and Liver of Developing Rats. The Journal of Nutrition 108:4, pages 585-594.
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D. Mitchell, C. Wagner, W.J. Stone, G.R. Wilkinson & S. Schenker. (1976) Abnormal Regulation of Plasma Pyridoxal 5'-Phosphate in Patients with Liver Disease. Gastroenterology 71:6, pages 1043-1049.
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WJ Stone, LG Warnock & C Wagner. (1975) Vitamin B6 deficiency in uremia. The American Journal of Clinical Nutrition 28:9, pages 950-957.
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B Shane & SF Contractor. (1975) Assessment of vitamin B6 status. Studies on pregnant women and oral contraceptive users. The American Journal of Clinical Nutrition 28:7, pages 739-747.
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Robert E. Cleary, Lawrence Lumeng & Ting-Kai Li. (1975) Maternal and fetal plasma levels of pyridoxal phosphate at term: Adequacy of vitamin B6 supplementation during pregnancy. American Journal of Obstetrics and Gynecology 121:1, pages 25-28.
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Lawrence Lumeng, Robert E. Cleary & Ting-Kai Li. (1974) Effect of oral contraceptives on the plasma concentration of pyridoxal phosphate. The American Journal of Clinical Nutrition 27:4, pages 326-333.
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Arne Hamfelt & Torsten Tuvemo. (1972) Pyridoxal phosphate and folic acid concentration in blood and erythrocyte aspartate aminotransferase activity during pregnancy. Clinica Chimica Acta 41, pages 287-298.
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C.E. Colombini & E.E. McCoy. (1970) Rapid thin-layer electrophoretic separation and estimation of all vitamin B6 compounds and of some 5-hydroxyindoles. Analytical Biochemistry 34:2, pages 451-458.
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Bruce Chabner & David Livingston. (1970) A simple enzymic assay for pyridoxal phosphate. Analytical Biochemistry 34:2, pages 413-423.
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Arne Hamfelt & Lennart Wetterberg. (1969) PYRIDOXAL PHOSPHATE IN ACUTE INTERMITTENT PORPHYRIA. Annals of the New York Academy of Sciences 166:1 Vitamin B6 in, pages 361-364.
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K. F. Gey & W. P. Burkard. (1969) CHLORPROMAZINE-INDUCED ACCUMULATION OF PYRIDOXAL-5'-PHOSPHATE AND ACTIVATION OF THE DECARBOXYLASE OF AROMATIC AMINO ACIDS IN RAT BRAIN. Annals of the New York Academy of Sciences 166:1 Vitamin B6 in, pages 213-224.
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Ernest Beutler. (1969) Glutathione Reductase: Stimulation in Normal Subjects by Riboflavin Supplementation. Science 165:3893, pages 613-615.
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Hitoshi Maruyama & David B. Coursin. (1968) Enzymic assay of pyridoxal phosphate using tyrosine apodecarboxylase and tyrosine-1-14C. Analytical Biochemistry 26:3, pages 420-429.
Crossref
George G. Guilbault. (2002) Use of enzymes in analytical chemistry. Analytical Chemistry 40:5, pages 459-471.
Crossref

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