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Research Article

A Simple Method to Establish Short-Term Cultures of Normal Human Colonic Epithelial Cells from Endoscopic Biopsy Specimens: Comparison of Isolation Methods, Assessment of Viability and Metabolic Activity

Pages 772-780 | Published online: 08 Jul 2009

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Read on this site (3)

Jakob B. Seidelin. (2015) Regulation of antiapoptotic and cytoprotective pathways in colonic epithelial cells in ulcerative colitis. Scandinavian Journal of Gastroenterology 50:sup1, pages 1-29.
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Casper Steenholdt, Lars Andresen, Gitte Pedersen, Alastair Hansen & Jørn Brynskov. (2009) Expression and function of toll-like receptor 8 and Tollip in colonic epithelial cells from patients with inflammatory bowel disease. Scandinavian Journal of Gastroenterology 44:2, pages 195-204.
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Mads Wichmann Matthiessen, Gitte Pedersen, Tatjana Albrektsen, Sven Adamsen, Jan Fleckner & Jørn Brynskov. (2005) Peroxisome proliferator-activated receptor expression and activation in normal human colonic epithelial cells and tubular adenomas. Scandinavian Journal of Gastroenterology 40:2, pages 198-205.
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Articles from other publishers (15)

John D. O’Neill, Meghan R. Pinezich, Brandon A. Guenthart & Gordana Vunjak-Novakovic. (2021) Gut bioengineering strategies for regenerative medicine. American Journal of Physiology-Gastrointestinal and Liver Physiology 320:1, pages G1-G11.
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Xiaohong Wang, Dan Li, Yong Zhang, Shuang Wu & Fang Tang. (2018) Costus root granules improve ulcerative colitis through regulation of TGF-β mediation of the PI3K/AKT signaling pathway. Experimental and Therapeutic Medicine.
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A. Wilhelm, F. Jahns, S. Böcker, H. Mothes, K.O. Greulich & M. Glei. (2012) Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells. Toxicology in Vitro 26:1, pages 133-141.
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Gitte Pedersen & Jørn Brynskov. (2010) Topical Rosiglitazone Treatment Improves Ulcerative Colitis by Restoring Peroxisome Proliferator-Activated Receptor-γ Activity. American Journal of Gastroenterology 105:7, pages 1595-1603.
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Andres I. Roig, Ugur Eskiocak, Suzie K. Hight, Sang Bum Kim, Oliver Delgado, Rhonda F. Souza, Stuart J. Spechler, Woodring E. Wright & Jerry W. Shay. (2010) Immortalized Epithelial Cells Derived From Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. Gastroenterology 138:3, pages 1012-1021.e5.
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G Pedersen, T Saermark, T Kirkegaard & J Brynskov. (2009) Spontaneous and cytokine induced expression and activity of matrix metalloproteinases in human colonic epithelium. Clinical and Experimental Immunology 155:2, pages 257-265.
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Matthew Bjerknes & Hazel Cheng. 2006. Adult Stem Cells. Adult Stem Cells 337 383 .
Dorina Rusu, Suzanne Loret, Olivier Peulen, Jacques Mainil & Guy Dandrifosse. (2005) Immunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures. BMC Cell Biology 6:1.
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G Pedersen, L Andresen, M W Matthiessen, J Rask-Madsen & J Brynskov. (2005) Expression of Toll-like receptor 9 and response to bacterial CpG oligodeoxynucleotides in human intestinal epithelium. Clinical and Experimental Immunology 141:2, pages 298-306.
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INGRID BARTSCH, INGRID ZSCHALER, MONIKA HASELOFF & PABLO STEINBERG. (2004) ESTABLISHMENT OF A LONG-TERM CULTURE SYSTEM FOR RAT COLON EPITHELIAL CELLS. In Vitro Cellular & Developmental Biology - Animal 40:8, pages 278.
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T KIRKEGAARD, G PEDERSEN, T SAERMARK & J BRYNSKOV. (2004) Tumour necrosis factor- α converting enzyme (TACE) activity in human colonic epithelial cells . Clinical and Experimental Immunology 135:1, pages 146-153.
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Jakob B. Seidelin, Thomas Horn & Ole H. Nielsen. (2003) Simple and efficient method for isolation and cultivation of endoscopically obtained human colonocytes. American Journal of Physiology-Gastrointestinal and Liver Physiology 285:6, pages G1122-G1128.
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Jiri Kalabis, Michael J. Patterson, Greg H. Enders, Brigitte Marian, Renato V. Iozzo, Gerhard Rogler, Phyllis A. Gimotty & Meenhard Herlyn. (2003) Stimulation of human colonic epithelial cells by leukemia inhibitory factor is dependent on collagen‐embedded fibroblasts in organotypic culture. The FASEB Journal 17:9, pages 1115-1117.
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Johannes Grossmann, Kathrin Walther, Monika Artinger, Stephan Kiessling, Martin Steinkamp, Wolf-Kuno Schmautz, Florian Stadler, Frauke Bataille, Michael Schultz, Jürgen Schölmerich & Gerhard Rogler. (2003) Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC). European Journal of Cell Biology 82:5, pages 262-270.
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G Pedersen, T Saermark, T Horn, B Giese, K Bendtzen & J Brynskov. (2000) CYTOKINE-INDUCED IMPAIRMENT OF SHORT-CHAIN FATTY ACID OXIDATION AND VIABILITY IN HUMAN COLONIC EPITHELIAL CELLS. Cytokine 12:9, pages 1400-1404.
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