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Xenobiotica
the fate of foreign compounds in biological systems
Volume 30, 2000 - Issue 2
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Research Article

Automated high throughput human CYP isoform activity assay using SPE-LC/MS method: application in CYP inhibition evaluation

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Pages 141-154 | Published online: 22 Sep 2008

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Yun-Jing Zhang, Wen-Li Zhou, Fei Yu, Qian Wang, Can Peng & Jia-Yi Kan. (2021) Evaluation of the effect of Bovis Calculus Artifactus on eight rat liver cytochrome P450 isozymes using LC-MS/MS and cocktail approach. Xenobiotica 51:9, pages 1010-1018.
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David J Greenblatt & Gary K Zammit. (2012) Pharmacokinetic evaluation of eszopiclone: clinical and therapeutic implications. Expert Opinion on Drug Metabolism & Toxicology 8:12, pages 1609-1618.
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James J Cali, Dongping Ma, Mary Sobol, Daniel J Simpson, Susan Frackman, Troy D Good, William J Daily & David Liu. (2006) Luminogenic cytochrome P450 assays. Expert Opinion on Drug Metabolism & Toxicology 2:4, pages 629-645.
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Slobodan Rendic. (2002) Summary of information on human CYP enzymes: human P450 metabolism data. Drug Metabolism Reviews 34:1-2, pages 83-448.
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J. Murray, D. Picking, A. Lamm, J. McKenzie, S. Hartley, C. Watson, L. Williams, H. Lowe & R. Delgoda. (2016) Significant inhibitory impact of dibenzyl trisulfide and extracts of Petiveria alliacea on the activities of major drug-metabolizing enzymes in vitro: An assessment of the potential for medicinal plant-drug interactions. Fitoterapia 111, pages 138-146.
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Valerie M. Kramlinger, Mónica Alvarado Rojas, Tatsuyuki Kanamori & F. Peter Guengerich. (2015) Cytochrome P450 3A Enzymes Catalyze the O6-Demethylation of Thebaine, a Key Step in Endogenous Mammalian Morphine Biosynthesis. Journal of Biological Chemistry 290:33, pages 20200-20210.
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Dipti B Ruikar & Sadhana J Rajput. (2012) Optimization of the in vitro oxidative biotransformation of glimepiride as a model substrate for cytochrome p450 using factorial design. DARU Journal of Pharmaceutical Sciences 20:1.
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Utkarsh Doshi & Albert P. Li. (2011) Luciferin IPA–Based Higher Throughput Human Hepatocyte Screening Assays for CYP3A4 Inhibition and Induction. SLAS Discovery 16:8, pages 903-909.
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Albert P. Li. (2009) Evaluation of Luciferin-Isopropyl Acetal as a CYP3A4 Substrate for Human Hepatocytes: Effects of Organic Solvents, Cytochrome P450 (P450) Inhibitors, and P450 Inducers. Drug Metabolism and Disposition 37:8, pages 1598-1603.
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Anima Ghosal, Ragulan Ramanathan, Narendra S. Kishnani, Swapan K. Chowdhury & Kevin B. Alton. 2005. Identification and Quantification of Drugs, Metabolites and Metabolizing Enzymes by LC-MS. Identification and Quantification of Drugs, Metabolites and Metabolizing Enzymes by LC-MS 295 336 .
Robert L. Walsky & R. Scott Obach. (2004) VALIDATED ASSAYS FOR HUMAN CYTOCHROME P450 ACTIVITIES. Drug Metabolism and Disposition 32:6, pages 647-660.
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. 2003. High Throughput Bioanalytical Sample Preparation - Methods and Automation Strategies. High Throughput Bioanalytical Sample Preparation - Methods and Automation Strategies 135 197 .
. 2003. High Throughput Bioanalytical Sample Preparation - Methods and Automation Strategies. High Throughput Bioanalytical Sample Preparation - Methods and Automation Strategies 41 74 .
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Takahito Yamamoto, Akio Suzuki & Yoshiro Kohno. (2002) Application of Microtiter Plate Assay to Evaluate Inhibitory Effects of Various Compounds on Nine Cytochrome P450 Isoforms and to Estimate their Inhibition Patterns. Drug Metabolism and Pharmacokinetics 17:5, pages 437-448.
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Hai-Zhi Bu, Kim Knuth, Lisa Magis & Philip Teitelbaum. (2001) High-throughput cytochrome P450 (CYP) inhibition screening via cassette probe-dosing strategy: III. Validation of a direct injection/on-line guard cartridge extraction−tandem mass spectrometry method for CYP2C19 inhibition evaluation. Journal of Pharmaceutical and Biomedical Analysis 25:3-4, pages 437-442.
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Hai‐Zhi Bu, Lisa Magis, Kim Knuth & Philip Teitelbaum. (2001) High‐throughput cytochrome P450 (CYP) inhibition screening via a cassette probe‐dosing strategy. VI. Simultaneous evaluation of inhibition potential of drugs on human hepatic isozymes CYP2A6, 3A4, 2C9, 2D6 and 2E1. Rapid Communications in Mass Spectrometry 15:10, pages 741-748.
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Ilona Kariv, Mark P. Fereshteh & Kevin R. Oldenburg. (2001) Development of a Miniaturized 384-Well High Throughput Screen for the Detection of Substrates of Cytochrome P450 2D6 and 3A4 Metabolism. SLAS Discovery 6:2, pages 91-99.
Crossref
Hai-Zhi Bu, Lisa Magis, Kim Knuth & Philip Teitelbaum. (2001) High-throughput cytochrome P450 (CYP) inhibition screening via cassette probe-dosing strategy. Journal of Chromatography B: Biomedical Sciences and Applications 753:2, pages 321-326.
Crossref
Hai-Zhi Bu, Kim Knuth, Lisa Magis & Philip Teitelbaum. (2001) High-throughput cytochrome P450 (CYP) inhibition screening via a cassette probe-dosing strategy. European Journal of Pharmaceutical Sciences 12:4, pages 447-452.
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Sheila J. Sadeghi, Georgia E. Tsotsou, Michael Fairhead, Yergalem T. Meharenna & Gianfranco Gilardi. 2000. Physics and Chemistry Basis of Biotechnology. Physics and Chemistry Basis of Biotechnology 71 104 .
L. Ramos, N. Brignol, R. Bakhtiar, T. Ray, L. M. Mc Mahon & F. L. S. Tse. (2000) High-throughput approaches to the quantitative analysis of ketoconazole, a potent inhibitor of cytochrome P450 3A4, in human plasma. Rapid Communications in Mass Spectrometry 14:23, pages 2282-2293.
Crossref
Hai-Zhi Bu, Kim Knuth, Lisa Magis & Philip Teitelbaum. (2000) High-throughput cytochrome P450 inhibition screening via cassette probe-dosing strategy. IV. Validation of a direct injection on-line guard cartridge extraction/tandem mass spectrometry method for simultaneous CYP3A4, 2D6 and 2E1 inhibition assessment. Rapid Communications in Mass Spectrometry 14:20, pages 1943-1948.
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Sean Ekins, Barbara J. Ring, James Grace, Donna J. McRobie-Belle & Steven A. Wrighton. (2000) Present and future in vitro approaches for drug metabolism. Journal of Pharmacological and Toxicological Methods 44:1, pages 313-324.
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