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Xenobiotica
the fate of foreign compounds in biological systems
Volume 33, 2003 - Issue 5
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Research Article

Prediction of in vivo hepatic clearance from in vitro data using cryopreserved human hepatocytes

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Pages 475-483 | Published online: 22 Sep 2008

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Read on this site (7)

Thierry Lavé, Kathryn Chapman, Paul Goldsmith & Malcolm Rowland. (2009) Human clearance prediction: shifting the paradigm. Expert Opinion on Drug Metabolism & Toxicology 5:9, pages 1039-1048.
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Nicola J. Hewitt, María José Gómez Lechón, J. Brian Houston, David Hallifax, Hayley S. Brown, Patrick Maurel, J. Gerald Kenna, Lena Gustavsson, Christina Lohmann, Christian Skonberg, Andre Guillouzo, Gregor Tuschl, Albert P. Li, Edward LeCluyse, Geny M. M. Groothuis & Jan G. Hengstler. (2007) Primary Hepatocytes: Current Understanding of the Regulation of Metabolic Enzymes and Transporter Proteins, and Pharmaceutical Practice for the Use of Hepatocytes in Metabolism, Enzyme Induction, Transporter, Clearance, and Hepatotoxicity Studies. Drug Metabolism Reviews 39:1, pages 159-234.
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Keith W Ward. (2005) Recent advances in pharmacokinetic extrapolation from preclinical data to humans. Expert Opinion on Drug Metabolism & Toxicology 1:4, pages 583-594.
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A.-K. Sohlenius-Sternbeck & S. Schmidt. (2005) Impaired glutathione-conjugating capacity by cryopreserved human and rat hepatocytes. Xenobiotica 35:7, pages 727-736.
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N Blanchard, E Alexandre, C. Abadie, T Lavé, B Heyd, G Mantion, D. Jaeck, L Richert & P Coassolo. (2005) Comparison of clearance predictions using primary cultures and suspensions of human hepatocytes. Xenobiotica 35:1, pages 1-15.
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E. Floby, S. Briem, Y. Terelius & A.-K. Sohlenius-Sternbeck. (2004) Use of a cocktail of probe substrates for drug-metabolizing enzymes for the assessment of the metabolic capacity of hepatocyte preparations. Xenobiotica 34:11-12, pages 949-959.
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Articles from other publishers (29)

Tsubasa Kameyama, Jasleen K. Sodhi & Leslie Z. Benet. (2022) Does Addition of Protein to Hepatocyte or Microsomal In Vitro Incubations Provide a Useful Improvement in In Vitro-In Vivo Extrapolation Predictability?. Drug Metabolism and Disposition 50:4, pages 401-412.
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L. J. Francis, J. B. Houston & D. Hallifax. (2021) Impact of Plasma Protein Binding in Drug Clearance Prediction: A Data Base Analysis of Published Studies and Implications for In Vitro-In Vivo Extrapolation. Drug Metabolism and Disposition 49:3, pages 188-201.
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Chuang Lu & Li Di. (2020) In vitro and in vivo methods to assess pharmacokinetic drug– drug interactions in drug discovery and development . Biopharmaceutics & Drug Disposition 41:1-2, pages 3-31.
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Karolina Słoczyńska, Agnieszka Gunia-Krzyżak, Paulina Koczurkiewicz, Katarzyna Wójcik-Pszczoła, Dorota Żelaszczyk, Justyna Popiół & Elżbieta Pękala. (2019) Metabolic stability and its role in the discovery of new chemical entities. Acta Pharmaceutica 69:3, pages 345-361.
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Kana Horiuchi, Shuichi Ohnishi, Takanobu Matsuzaki, Satoko Funaki, Ayahisa Watanabe, Tohru Mizutare, Sayaka Matsumoto, Ken-ichi Nezasa & Hiroshi Hasegawa. (2018) Improved Human Pharmacokinetic Prediction of Hepatically Metabolized Drugs With Species-Specific Systemic Clearance. Journal of Pharmaceutical Sciences 107:5, pages 1443-1453.
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Khaled A. Shibany, Sabine Tötemeyer, Stefanie L. Pratt & Stuart W. Paine. (2016) Equine hepatocytes: isolation, cryopreservation, and applications to in vitro drug metabolism studies. Pharmacology Research & Perspectives 4:5.
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Chuang Lu. 2014. Handbook of Metabolic Pathways of Xenobiotics. Handbook of Metabolic Pathways of Xenobiotics 1 23 .
Patricio Godoy, Nicola J. Hewitt, Ute Albrecht, Melvin E. Andersen, Nariman Ansari, Sudin Bhattacharya, Johannes Georg Bode, Jennifer Bolleyn, Christoph Borner, Jan Böttger, Albert Braeuning, Robert A. Budinsky, Britta Burkhardt, Neil R. Cameron, Giovanni Camussi, Chong-Su Cho, Yun-Jaie Choi, J. Craig Rowlands, Uta Dahmen, Georg Damm, Olaf Dirsch, María Teresa Donato, Jian Dong, Steven Dooley, Dirk Drasdo, Rowena Eakins, Karine Sá Ferreira, Valentina Fonsato, Joanna Fraczek, Rolf Gebhardt, Andrew Gibson, Matthias Glanemann, Chris E. P. Goldring, María José Gómez-Lechón, Geny M. M. Groothuis, Lena Gustavsson, Christelle Guyot, David Hallifax, Seddik Hammad, Adam Hayward, Dieter Häussinger, Claus Hellerbrand, Philip Hewitt, Stefan Hoehme, Hermann-Georg Holzhütter, J. Brian Houston, Jens Hrach, Kiyomi Ito, Hartmut Jaeschke, Verena Keitel, Jens M. Kelm, B. Kevin Park, Claus Kordes, Gerd A. Kullak-Ublick, Edward L. LeCluyse, Peng Lu, Jennifer Luebke-Wheeler, Anna Lutz, Daniel J. Maltman, Madlen Matz-Soja, Patrick McMullen, Irmgard Merfort, Simon Messner, Christoph Meyer, Jessica Mwinyi, Dean J. Naisbitt, Andreas K. Nussler, Peter Olinga, Francesco Pampaloni, Jingbo Pi, Linda Pluta, Stefan A. Przyborski, Anup Ramachandran, Vera Rogiers, Cliff Rowe, Celine Schelcher, Kathrin Schmich, Michael Schwarz, Bijay Singh, Ernst H. K. Stelzer, Bruno Stieger, Regina Stöber, Yuichi Sugiyama, Ciro Tetta, Wolfgang E. Thasler, Tamara Vanhaecke, Mathieu Vinken, Thomas S. Weiss, Agata Widera, Courtney G. Woods, Jinghai James Xu, Kathy M. Yarborough & Jan G. Hengstler. (2013) Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Archives of Toxicology 87:8, pages 1315-1530.
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Donglu Zhang, Gang Luo, Xinxin Ding & Chuang Lu. (2012) Preclinical experimental models of drug metabolism and disposition in drug discovery and development. Acta Pharmaceutica Sinica B 2:6, pages 549-561.
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Gang Luo, Chuang Lu, Xinxin Ding & Donglu Zhang. 2011. Mass Spectrometry in Drug Metabolism and Disposition. Mass Spectrometry in Drug Metabolism and Disposition 151 196 .
Zichao Rao, Luqin Si, Yanbin Guan, Hongping Pan, Jun Qiu & Gao Li. (2010) Inhibitive effect of cremophor RH40 or tween 80-based self-microemulsiflying drug delivery system on cytochrome P450 3A enzymes in murine hepatocytes. Journal of Huazhong University of Science and Technology [Medical Sciences] 30:5, pages 562-568.
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M. Teresa Donato, David Hallifax, Laura Picazo, José V. Castell, J. Brian Houston, M. José Gomez-Lechón & Agustin Lahoz. (2010) Metabolite Formation Kinetics and Intrinsic Clearance of Phenacetin, Tolbutamide, Alprazolam, and Midazolam in Adenoviral Cytochrome P450-Transfected HepG2 Cells and Comparison with Hepatocytes and In Vivo. Drug Metabolism and Disposition 38:9, pages 1449-1455.
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Paulo Paixão, Luís F. Gouveia & José A.G. Morais. (2010) Prediction of the in vitro intrinsic clearance determined in suspensions of human hepatocytes by using artificial neural networks. European Journal of Pharmaceutical Sciences 39:5, pages 310-321.
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Urban Fagerholm. (2007) Prediction of human pharmacokinetics—evaluation of methods for prediction of hepatic metabolic clearance. Journal of Pharmacy and Pharmacology 59:6, pages 803-828.
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Nadège Blanchard, Nicola J Hewitt, Paul Silber, Hannah Jones, Philippe Coassolo & Thierry Lave. (2006) Prediction of hepatic clearance using cryopreserved human hepatocytes: a comparison of serum and serum-free incubations. Journal of Pharmacy and Pharmacology 58:5, pages 633-641.
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Paulo Paixão, Luís F. Gouveia & José A.G. Morais. (2009) Prediction of drug distribution within blood. European Journal of Pharmaceutical Sciences 36:4-5, pages 544-554.
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Xing Han, Robert T. Mingoia, Diane L. Nabb, Ching-Hui Yang, Suzanne I. Snajdr & Robert A. Hoke. (2008) Xenobiotic intrinsic clearance in freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss): Determination of trout hepatocellularity, optimization of cell concentrations and comparison of serum and serum-free incubations. Aquatic Toxicology 89:1, pages 11-17.
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Chuang Lu, Panos Hatsis, Cicely Berg, Frank W. Lee & Suresh K. Balani. (2008) Prediction of Pharmacokinetic Drug-Drug Interactions Using Human Hepatocyte Suspension in Plasma and Cytochrome P450 Phenotypic Data. II. In Vitro-in Vivo Correlation with Ketoconazole. Drug Metabolism and Disposition 36:7, pages 1255-1260.
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Christine Huang, Ming Zheng, Zheng Yang, A. David Rodrigues & Punit Marathe. (2007) Projection of Exposure and Efficacious Dose Prior to First-in-Human Studies: How Successful Have We Been?. Pharmaceutical Research 25:4, pages 713-726.
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Punit H. Marathe, Christine Huang & A. David Rodrigues. 2008. 311 328 .
Matthew G. Soars, Dermot F. McGinnity, Ken Grime & Robert J. Riley. (2007) The pivotal role of hepatocytes in drug discovery. Chemico-Biological Interactions 168:1, pages 2-15.
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Chuang Lu, Gerald T. Miwa, Shimoga R. Prakash, Liang-Shang Gan & Suresh K. Balani. (2007) A Novel Model for the Prediction of Drug-Drug Interactions in Humans Based on in Vitro Cytochrome P450 Phenotypic Data. Drug Metabolism and Disposition 35:1, pages 79-85.
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Larry J. Jolivette & Sean Ekins. 2007. 131 176 .
Chuang Lu, Ping Li, Richard Gallegos, Vinita Uttamsingh, Cindy Q. Xia, Gerald T. Miwa, Suresh K. Balani & Liang-Shang Gan. (2006) Comparison of Intrinsic Clearance in Liver Microsomes and Hepatocytes from Rats and Humans: Evaluation of Free Fraction and Uptake in Hepatocytes. Drug Metabolism and Disposition 34:9, pages 1600-1605.
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Delphine Jouin, Nadège Blanchard, Eliane Alexandre, Frédéric Delobel, Pascale David-Pierson, Thierry Lavé, Daniel Jaeck, Lysiane Richert & Philippe Coassolo. (2006) Cryopreserved human hepatocytes in suspension are a convenient high throughput tool for the prediction of metabolic clearance. European Journal of Pharmaceutics and Biopharmaceutics 63:3, pages 347-355.
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Inge A. M. de Graaf, Ruben de Kanter, Marina H. de Jager, Raquel Camacho, Elise Langenkamp, Esther G. van de Kerkhof & Geny M. M. Groothuis. (2006) EMPIRICAL VALIDATION OF A RAT IN VITRO ORGAN SLICE MODEL AS A TOOL FOR IN VIVO CLEARANCE PREDICTION. Drug Metabolism and Disposition 34:4, pages 591-599.
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Kenneth Bachmann, Urvi Telang, James Byers & Wayne Hoss. (2005) The Processing of the Selective M1 Agonist CDD-0102-J by Human Hepatic Drug Metabolizing Enzymes. American Journal of Therapeutics 12:4, pages 300-305.
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Dermot F. McGinnity, Matthew G. Soars, Richard A. Urbanowicz & Robert J. Riley. (2004) EVALUATION OF FRESH AND CRYOPRESERVED HEPATOCYTES AS IN VITRO DRUG METABOLISM TOOLS FOR THE PREDICTION OF METABOLIC CLEARANCE. Drug Metabolism and Disposition 32:11, pages 1247-1253.
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Nick Plant. (2004) Strategies for using in vitro screens in drug metabolism. Drug Discovery Today 9:7, pages 328-336.
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