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Original Articles

Development of a specific radioimmunoassay for the detection of clenbuterol residues in treated cattle

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Pages 43-53 | Accepted 19 Jul 1990, Published online: 10 Jan 2009

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R.H.M.M. Granja, A.M. Montes Niño, F. Rabone, R.E. Montes Niño, A. Cannavan & A. Gonzalez Salerno. (2008) Validation of radioimmunoassay screening methods for β-agonists in bovine liver according to Commission Decision 2002/657/EC. Food Additives & Contaminants: Part A 25:12, pages 1475-1481.
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Ting Xu, BaoM. Wang, Wei Sheng, QingX. Li, XiaoL. Shao & Ji Li. (2007) Application of an enzyme-linked immunosorbent assay for the detection of clenbuterol residues in swine urine and feeds. Journal of Environmental Science and Health, Part B 42:2, pages 173-177.
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Weilin L. Shelver & David J. Smith. (2000) Evaluation of commercial immunoassays for cross-reactivity to clenbuterol stereoisomers and bovine metabolites. Food Additives & Contaminants 17:10, pages 837-845.
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Sabine Hahnau & Bernd Jülicher. (1996) Evaluation of commercially available ELISA test kits for the detection of clenbuterol and other β2‐agonists. Food Additives & Contaminants 13:3, pages 259-274.
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Eleonora Petruzzelli, Adriano Ius, Silvia Berta, Mauro Dovis & Alberto Albertini. (1996) Preparation and characterization of a monoclonal antibody specific for the β‐agonist clenbuterol. Food and Agricultural Immunology 8:1, pages 3-10.
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M.‐P. Montrade, B. Le Bizec, F. Monteau & F. Andre. (1995) Analysis of β‐agonists in urine and tissues by capillary gas chromatography‐mass spectrometry: In vivo study of salbutamol disposition in calves. Food Additives & Contaminants 12:5, pages 625-636.
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K. Vanoosthuyze, C. Van Peteghem, D. Courtheyn & J. Vercammen. (1994) Use of an immunomagnetic separation‐ELISA technique for the fast detection of growth promoters in cattle urine. Food and Agricultural Immunology 6:3, pages 241-249.
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C. T. Elliott, W. J. McCaughey & H. D. Shortt. (1993) Residues of the beta‐agonist clenbuterol in tissues of medicated farm animals. Food Additives & Contaminants 10:2, pages 231-244.
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M. Paleologo Oriundi, G. Giacomini, F. Ballaben, F. Berti, F. Benedetti, R. Bagnati & E. Bastiani. (1992) An enzyme‐linked immunosorbent assay for the direct analysis of beta‐agonist drugs in urine and sera. Food and Agricultural Immunology 4:2, pages 73-82.
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Articles from other publishers (29)

Shuyu Ouyang, Shuting Yu & Yingying Le. (2022) Current Advances in Immunoassays for the Detection of β2-Agonists. Foods 11:6, pages 803.
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Fernando X. Moreira, Renata Silva, Maria B. André, Paula G. de Pinho, Maria L. Bastos, João Ruivo, Patrícia Ruivo & Helena Carmo. (2018) Quantification of doping compounds in faecal samples from racing pigeons, by liquid chromatography-tandem mass spectrometry. Journal of Chromatography B 1089, pages 33-42.
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LiQi Wang, ZhenLing Zeng, Zhong Wang, JianYing Guo & LiMin He. (2015) Influence of Water in Samples on Residues Analysis of Beta-Agonists in Porcine Tissues and Urine Using Liquid Chromatography Tandem Mass Spectrometry. Food Analytical Methods 9:7, pages 1904-1911.
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LiQi WangZhenLing Zeng, Zhong Wang & LiMin He. (2016) Correction function for biased results due to matrix effects in residue analysis of beta-agonists in porcine tissues and urine with LC-MS/MS. RSC Advances 6:32, pages 26967-26976.
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Ling Wang, Ran Yang, Jing Chen, Jianjun Li, Lingbo Qu & Peter de B. Harrington. (2014) Sensitive voltammetric sensor based on Isopropanol–Nafion–PSS–GR nanocomposite modified glassy carbon electrode for determination of Clenbuterol in pork. Food Chemistry 164, pages 113-118.
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D. Mauro, S. Ciardullo, C. Civitareale, M. Fiori, A.A. Pastorelli, P. Stacchini & G. Palleschi. (2014) Development and validation of a multi-residue method for determination of 18 β-agonists in bovine urine by UPLC–MS/MS. Microchemical Journal 115, pages 70-77.
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A.G. Fragkaki, C. Georgakopoulos, S. Sterk & M.W.F. Nielen. (2013) Sports doping: Emerging designer and therapeutic β2-agonists. Clinica Chimica Acta 425, pages 242-258.
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Liqi Wang, Zhenling Zeng, Xufeng Wang, Jianwen Yang, Zhaohua Chen & Limin He. (2013) Multiresidue analysis of nine β-agonists in animal muscles by LC-MS/MS based on a new polymer cartridge for sample cleanup. Journal of Separation Science 36:11, pages 1843-1852.
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Fang Yang, Zhencai Liu, Yonghui Lin, Jian Chen, Guofang Pang & Guonan Chen. (2011) Development an UHPLC-MS/MS Method for Detection of β-Agonist Residues in Milk. Food Analytical Methods 5:1, pages 138-147.
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C. Juan, C. Igualada, F. Moragues, N. León & J. Mañes. (2010) Development and validation of a liquid chromatography tandem mass spectrometry method for the analysis of β-agonists in animal feed and drinking water. Journal of Chromatography A 1217:39, pages 6061-6068.
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Bing Shao, Xiaofei Jia, Jing Zhang, Juan Meng, Yongning Wu, Hejun Duan & Xiaoming Tu. (2009) Multi-residual analysis of 16 β-agonists in pig liver, kidney and muscle by ultra performance liquid chromatography tandem mass spectrometry. Food Chemistry 114:3, pages 1115-1121.
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Sophie Danyi, Guy Degand, Colette Duez, Benoît Granier, Guy Maghuin-Rogister & Marie-Louise Scippo. (2007) Solubilisation and binding characteristics of a recombinant β2-adrenergic receptor expressed in the membrane of Escherichia coli for the multianalyte detection of β-agonists and antagonists residues in food-producing animals. Analytica Chimica Acta 589:2, pages 159-165.
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G.P. Zhang, X.N. Wang, J.F. Yang, Y.Y. Yang, G.X. Xing, Q.M. Li, D. Zhao, S.J. Chai & J.Q. Guo. (2006) Development of an immunochromatographic lateral flow test strip for detection of β-adrenergic agonist Clenbuterol residues. Journal of Immunological Methods 312:1-2, pages 27-33.
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Andrej Preželj, Janez Štrancar, Franc Gubenšek, Slavko Pečar & Guy Degand. (2003) Quantification of binding of some thiol-reactive clenbuterol analogues to bovine serum albumin by electron paramagnetic resonance spectroscopy. Analytical Biochemistry 315:2, pages 202-207.
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Y Martı́n. (2002) Influence of the storage conditions on the concentration of clenbuterol in cattle urine samples. Analytica Chimica Acta 452:1, pages 115-122.
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Adriano Podestà, Alberto Luppi, Leonardo Benatti, Chiara Villani, Giorgio Montagnoli & Franco Martelli. (1998) A source of false-negative results in clenbuterol analysis in tissues of veal calves. Journal of Pharmacological and Toxicological Methods 40:1, pages 27-32.
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Daniel Zalko, Georges Bories & Jacques Tulliez. (1998) Metabolic Fate of Clenbuterol in Calves. Journal of Agricultural and Food Chemistry 46:5, pages 1935-1943.
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Jenny Cai & Jack Henion. (1997) Quantitative multi-residue determination of β-agonists in bovine urine using on-line immunoaffinity extraction-coupled column packed capillary liquid chromatography-tandem mass spectrometry. Journal of Chromatography B: Biomedical Sciences and Applications 691:2, pages 357-370.
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NEIL T. CROSBY. 1997. Determination of Veterinary Residues in Food. Determination of Veterinary Residues in Food 148 176 .
Peter Batjoens, Dirk Courtheyn, Hubert F. De Brabander, Jan Vercammen, Katia De Wasch & Maureen Logghe. (1996) Gas chromatographic-tandem mass spectrometric analysis of clenbuterol residues in faeces. Journal of Chromatography A 750:1-2, pages 133-139.
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Damien Boyd, Michael O'Keeffe & Malcolm R. Smyth. (1996) Methods for the determination of β-agonists in biological matrices. A review. The Analyst 121:1, pages 1R-10R.
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Carmen Gabiola, María A. García‐Calonge, María P. Portillo, J. Alfredo Martínez & Antonio S. del Barrio. (1998) Validation of a method for the determination of salbutamol in animal urine by gas chromatography‐mass spectrometry and its application to treated lamb samples. Journal of Microcolumn Separations 8:5, pages 361-364.
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M.J. SAUER, R.J.H. PICKETT, S. LIMER & S.N. DIXON. (2008) Distribution and elimination of clenbuterol in tissues and fluids of calves following prolonged oral administration at a growth‐promoting dose. Journal of Veterinary Pharmacology and Therapeutics 18:2, pages 81-86.
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J. Cartañà, T. Segués, M. Yebras, N.J. Rothwell & M.J. Stock. (1994) Anabolic effects of clenbuterol after long-term treatment and withdrawal in the rat. Metabolism 43:9, pages 1086-1092.
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W. John Blanchflower, S. Armstrong Hewitt, A. Cannavan, Christopher T. Elliott & D. Glenn Kennedy. (1993) Detection of clenbuterol residues in bovine liver, muscle, retina and urine using gas chromatography/mass spectrometry. Biological Mass Spectrometry 22:6, pages 326-330.
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R.J.H. Pickett & M.J. Sauer. (1993) Determination of clenbuterol in bovine urine by enzyme immunoassay following concentration and clean-up by immunoaffinity chromatography. Analytica Chimica Acta 275:1-2, pages 269-273.
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M.-P. Montrade, B. Le Bizec, F. Monteau, B. Siliart & F. Andre. (1993) Multi-residue analysis for β-agonistic drugs in urine of meat-producing animals by gas chromatography—mass spectrometry. Analytica Chimica Acta 275:1-2, pages 253-268.
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D. Boyd, P. Shearan, J.P. Hopkins, M. O'Keeffe & M.R. Smyth. (1993) Application of matrix solid phase dispersion for the determination of clenbuterol in liver samples. Analytica Chimica Acta 275:1-2, pages 221-226.
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Maurice J. Sauer, Robert J.H. Pickett & A. Louise MacKenzie. (1993) Determination of clenbuterol residues in bovine liver, urine, and eye by enzyme immunoassay. Analytica Chimica Acta 275:1-2, pages 195-203.
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