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EMERGING TECHNOLOGIES

Multiple virus and viroid detection and strain separation via multiplex reverse transcription – polymerase chain reaction

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Pages 127-134 | Accepted 15 Apr 2003, Published online: 01 Apr 2010

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Xianzhou Nie, Rudra P. Singh & Mathuresh Singh. (2004) Molecular and pathological characterization of N:O isolates of the Potato virus Y from Manitoba, Canada. Canadian Journal of Plant Pathology 26:4, pages 573-583.
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Articles from other publishers (18)

Xianzhou Nie, Mathuresh Singh, Dahu Chen, Cassandra Gilchrist, Yasmine Soqrat, Manisha Shukla, Alexa Creelman, Virginia Dickison, Bihua Nie, Jacques Lavoie & Vikram Bisht. (2021) Development of High-Resolution DNA Melting Analysis for Simultaneous Detection of Potato Mop-Top Virus and Its Vector, Spongospora subterranea , in Soil . Plant Disease 105:4, pages 948-957.
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Wei Zhang, Zhixiang Zhang, Guoquan Fan, Yanling Gao, Jingzhi Wen, Yanju Bai, Cailing Qiu, Shu Zhang, Yu Shen & Xianxin Meng. (2016) Development and application of a universal and simplified multiplex RT-PCR assay to detect five potato viruses. Journal of General Plant Pathology 83:1, pages 33-45.
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Bogumiła Zacharzewska, Agnieszka Przewodowska & Krzysztof Treder. (2014) The Adaptation of Silica Capture RT-PCR for the Detection of Potato Virus Y. American Journal of Potato Research 91:5, pages 525-531.
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Yongjiang Zhang, Jun Yin, Dongmei Jiang, Yanyan Xin, Fang Ding, Ziniu Deng, Guoping Wang, Xianfeng Ma, Fang Li, Guifen Li, Mingfu Li, Shifang Li & Shuifang Zhu. (2013) A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level. PLoS ONE 8:5, pages e64474.
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Mathuresh Singh, Rudra P. Singh, M. S. Fageria, X. Nie, Robert Coffin & G. Hawkins. (2012) Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers. American Journal of Potato Research 90:1, pages 43-50.
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K. Subramanya SastryK. Subramanya Sastry. 2013. Plant Virus and Viroid Diseases in the Tropics. Plant Virus and Viroid Diseases in the Tropics 233 353 .
Sandeep Kumar, V. K. Baranwal, Subodh Joshi, Meenakshi Arya & S. Majumder. (2010) Simultaneous Detection of Mixed Infection of Onion yellow dwarf virus and an Allexivirus in RT-PCR for Ensuring Virus Free Onion Bulbs. Indian Journal of Virology 21:1, pages 64-68.
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Xinxi Hu, Changzheng He, Ya Xiao, Xingyao Xiong & Xianzhou Nie. (2009) Molecular characterization and detection of recombinant isolates of potato virus Y from China. Archives of Virology 154:8, pages 1303-1312.
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S. Sadeghi, M. Hedayati & S. Jamili. (2009) Determination of Sperms’ ATP Content of Golden Grey Mullet (Mugil auratus) at Different Conditions. Biotechnology(Faisalabad) 8:3, pages 375-379.
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L. Imanparast & D. Hassanpana. (2009) Response of Onobrychis Genotypes to PEG 10000 Induced Osmotic Stress. Biotechnology(Faisalabad) 8:3, pages 365-369.
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M. S. Khan, C. Timmermann, M. I. Hoque, R. H. Sarker & H.-P. Mühlbach. (2016) Detection of potato spindle tuber viroid (PSTVd) in minute amounts of potato (Solanum tuberosum L.) leaf tissue by hybridization techniques and, together with potato viruses, by multiplex RT-PCRNachweis des Kartoffelspindelknollenviroids (PSTVd) in Kleinstmengen von Kartoffelblattgewebe (Solanum tuberosum L.) durch Hybridisierungstechniken und, zusammen mit Kartoffelviren, durch Multiplex-RT-PCR. Journal of Plant Diseases and Protection 116:3, pages 97-105.
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Xianzhou Nie, Yanju Bai, Teresa A. Molen & David C. Desjardins. (2008) Development of universal primers for detection of potato carlaviruses by RT-PCR. Journal of Virological Methods 149:2, pages 209-216.
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Jan H.W. Bergervoet, Jeroen Peters, José R.C.M. van Beckhoven, Gé W. van den Bovenkamp, James W. Jacobson & Jan M. van der Wolf. (2008) Multiplex microsphere immuno-detection of potato virus Y, X and PLRV. Journal of Virological Methods 149:1, pages 63-68.
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R. Viswanathan, M. Balamuralikrishnan & R. Karuppaiah. (2008) Duplex — reverse transcription — polymerase chain reaction (D-RT-PCR)-a technique for the simultaneous detection of viruses causing sugarcane mosaic. Sugar Tech 10:1, pages 81-86.
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Bright O. Agindotan, Patrick J. Shiel & Philip H. Berger. (2007) Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan® real-time RT-PCR. Journal of Virological Methods 142:1-2, pages 1-9.
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M. Peiman & C. Xie. (2006) Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber. Australasian Plant Disease Notes 1:1, pages 41.
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Rudra P. Singh, Andrea D. Dilworth, Mathuresh Singh & Debra L. McLaren. (2004) Evaluation of a simple membrane-based nucleic acid preparation protocol for RT–PCR detection of potato viruses from aphid and plant tissues. Journal of Virological Methods 121:2, pages 163-170.
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H. Bostan .K. Haliloglu .. (2004) Distribution of PLRV, PVS, PVX and PVY (PVYN, PVYo and PVYc) in the Seed Potato Tubers in Turkey. Pakistan Journal of Biological Sciences 7:7, pages 1140-1143.
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