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Canadian Journal of Plant Pathology Volume 37, 2015 - Issue 3
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Epidemiology/Épidémiologie

Pseudoperonospora cubensis and P. humuli detection using species-specific probes and high definition melt curve analysis

Carly F. SummersPlant Pathology and Plant-Microbe Biology Section, SIPS, Cornell University, Geneva, NY14456, USAView further author information
,
Nanci L. AdairUSDA-ARS, Forage Seed and Cereal Research Unit and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR97330, USAView further author information
,
David H. GentUSDA-ARS, Forage Seed and Cereal Research Unit and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR97330, USAView further author information
,
Margaret T. McGrathPlant Pathology and Plant-Microbe Biology Section, SIPS, Cornell University, Long Island Horticultural Research and Extension Center, Riverhead, NY11901, USAView further author information
&
Christine D. SmartPlant Pathology and Plant-Microbe Biology Section, SIPS, Cornell University, Geneva, NY14456, USACorrespondence[email protected]
View further author information
Pages 315-330 | Accepted 16 May 2015, Published online: 08 Jun 2015

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Dong Jae Lee, Jae Sung Lee & Young-Joon Choi. (2021) Co-Occurrence of Two Phylogenetic Clades of Pseudoperonospora cubensis, the Causal Agent of Downy Mildew Disease, on Oriental Pickling Melon. Mycobiology 49:2, pages 188-195.
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Alamgir Rahman, Timothy D. Miles, Frank N. Martin & Lina M. Quesada-Ocampo. (2017) Molecular approaches for biosurveillance of the cucurbit downy mildew pathogen, Pseudoperonospora cubensis. Canadian Journal of Plant Pathology 39:3, pages 282-296.
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Articles from other publishers (31)

Marcus O. Olatoye, Michele Wiseman, David H. Gent, John A. Henning & Kayla R. Altendorf. (2023) Genetic characterization of downy mildew resistance from the hop ( Humulus lupulus L.) line USDA 64035M . Crop Science 63:3, pages 1082-1091.
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Walter F. Mahaffee, Fabien Margairaz, Lucas Ulmer, Brian N. Bailey & Rob Stoll. (2023) Catching Spores: Linking Epidemiology, Pathogen Biology, and Physics to Ground-Based Airborne Inoculum Monitoring. Plant Disease 107:1, pages 13-33.
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Douglas S. Higgins, Timothy D. Miles, Jan M. Byrne & Mary K. Hausbeck. (2022) Optimizing Molecular Detection for the Hop Downy Mildew Pathogen Pseudoperonospora humuli in Plant Tissue . Phytopathology® 112:11, pages 2426-2439.
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Julian C. Bello, Douglas S. Higgins, Monique L. Sakalidis, Lina M. Quesada-Ocampo, Frank Martin & Mary K. Hausbeck. (2022) Clade-Specific Monitoring of Airborne Pseudoperonospora spp. Sporangia Using Mitochondrial DNA Markers for Disease Management of Cucurbit Downy Mildew . Phytopathology® 112:10, pages 2110-2125.
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J. R. Standish, E. Góngora-Castillo, M. J. BowmanK. L. ChildsM. TianL. M. Quesada-Ocampo. (2022) Development, Validation, and Utility of Species-Specific Diagnostic Markers for Detection of Peronospora belbahrii . Phytopathology® 112:8, pages 1667-1675.
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Hervé Van der Heyden, Pierre Dutilleul, Marc‐Olivier Duceppe, Guillaume J. Bilodeau, Jean‐Benoît Charron & Odile Carisse. (2021) Genotyping by sequencing suggests overwintering of Peronospora destructor in southwestern Québec, Canada . Molecular Plant Pathology 23:3, pages 339-354.
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Sharifa G. Crandall, Marina L. Ramon, Alyssa K. Burkhardt, Julian Camilo Bello Rodriguez, Nanci Adair, David H. Gent, Mary K. Hausbeck, Lina M. Quesada-Ocampo & Frank N. Martin. (2021) A Multiplex TaqMan qPCR Assay for Detection and Quantification of Clade 1 and Clade 2 Isolates of Pseudoperonospora cubensis and Pseudoperonospora humuli . Plant Disease 105:10, pages 3154-3161.
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Marcin Nowicki, Denita Hadziabdic, Robert N. Trigiano, Sarah L. Boggess, Loukas Kanetis, Phillip A. Wadl, Peter S. Ojiambo, Marc A. Cubeta, Otmar Spring, Marco Thines, Fabian Runge & Brian E. Scheffler. (2021) “Jumping Jack”: Genomic Microsatellites Underscore the Distinctiveness of Closely Related Pseudoperonospora cubensis and Pseudoperonospora humuli and Provide New Insights Into Their Evolutionary Past. Frontiers in Microbiology 12.
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Savithri Purayannur, David H. Gent, Timothy D. Miles, Sebastjan Radišek & Lina M. Quesada‐Ocampo. (2021) The hop downy mildew pathogen Pseudoperonospora humuli . Molecular Plant Pathology 22:7, pages 755-768.
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Hervé Van der Heyden, Pierre Dutilleul, Jean-Benoit Charron, Guillaume J. Bilodeau & Odile Carisse. (2021) Monitoring airborne inoculum for improved plant disease management. A review. Agronomy for Sustainable Development 41:3.
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Douglas S. Higgins & Mary K. Hausbeck. (2021) Susceptibility of Hop Cultivars and Rootstock to Downy Mildew Caused by Pseudoperonospora humuli. HortScience 56:5, pages 543-550.
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Julian C. Bello, Monique L. Sakalidis, David E. Perla & Mary K. Hausbeck. (2021) Detection of Airborne Sporangia of Pseudoperonospora cubensis and P . humuli in Michigan Using Burkard Spore Traps Coupled to Quantitative PCR . Plant Disease, pages PDIS-07-20-1534.
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Miloslav Kitner, Fabian Runge, Aleš Lebeda, Lucie Vaculná, Božena Sedláková & Marco Thines. (2021) Pseudoperonospora humuli might be an introduced species in Central Europe with low genetic diversity but high distribution potential. European Journal of Plant Pathology 159:4, pages 903-915.
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Andres F. Salcedo, Savithri Purayannur, Jeffrey R. Standish, Timothy Miles, Lindsey Thiessen & Lina M. Quesada-Ocampo. (2021) Fantastic Downy Mildew Pathogens and How to Find Them: Advances in Detection and Diagnostics. Plants 10:3, pages 435.
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A. Rahman, J. R. Standish, K. N. D’Arcangelo & L. M. Quesada-Ocampo. (2021) Clade-Specific Biosurveillance of Pseudoperonospora cubensis Using Spore Traps for Precision Disease Management of Cucurbit Downy Mildew . Phytopathology® 111:2, pages 312-320.
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Timothy D. Miles, Tara M. Neill, Marivi Colle, Brent Warneke, Guy Robinson, Ioannis Stergiopoulos & Walter F. Mahaffee. (2021) Allele-Specific Detection Methods for QoI Fungicide-Resistant Erysiphe necator in Vineyards . Plant Disease 105:1, pages 175-182.
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Kishore Babu Bandamaravuri, Ashish K. Nayak, Anu Sharma Bandamaravuri & Abdul Samad. (2020) Simultaneous detection of downy mildew and powdery mildew pathogens on Cucumis sativus and other cucurbits using duplex-qPCR and HRM analysis. AMB Express 10:1.
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E. C. Wallace, K. N. D’Arcangelo & L. M. Quesada-Ocampo. (2020) Population Analyses Reveal Two Host-Adapted Clades of Pseudoperonospora cubensis , the Causal Agent of Cucurbit Downy Mildew, on Commercial and Wild Cucurbits . Phytopathology® 110:9, pages 1578-1587.
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. (2020) Full Issue PDF. Plant Health Progress 21:3, pages 157-223.
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Andres Salcedo, Mary Hausbeck, Stacey Pigg & Lina M. Quesada-Ocampo. (2020) Diagnostic Guide for Cucurbit Downy Mildew. Plant Health Progress 21:3, pages 166-172.
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Savithri Purayannur, Timothy D. Miles, David H. Gent, Stacey Pigg & Lina M. Quesada-Ocampo. (2020) Hop Downy Mildew Caused by Pseudoperonospora humuli : A Diagnostic Guide . Plant Health Progress 21:3, pages 173-179.
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Anthony P. Keinath, Sally A. Miller & Christine D. Smart. (2019) Response of Pseudoperonospora cubensis to Preventative Fungicide Applications Varies by State and Year . Plant Health Progress 20:3, pages 142-146.
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Bruna B. Forcelini, Seonghee LeeMichelle S. OliveiraNatalia A. Peres. (2018) Development of High-Throughput SNP Genotyping Assays for Rapid Detection of Strawberry Colletotrichum Species and the G143A Mutation . Phytopathology® 108:12, pages 1501-1508.
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Prathibha Veerappa Hanumanthappa, Vinayaka Hegde, Sharadraj Kuriyathadka Mahalingeshwara, Rajesh Muliyar Krishna, Rachana Kaitheri Edathil & Chowdappa Pallem. (2018) Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis. Journal of Plant Pathology 100:2, pages 233-240.
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Amy M. Baetsen-Young, Matthew Vasher, Leann L. Matta, Phil Colgan, Evangelyn C. Alocilja & Brad Day. (2018) Direct colorimetric detection of unamplified pathogen DNA by dextrin-capped gold nanoparticles. Biosensors and Bioelectronics 101, pages 29-36.
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Sharifa G. Crandall, Alamgir Rahman, Lina M. Quesada-Ocampo, Frank N. Martin, Guillaume J. Bilodeau & Timothy D. Miles. (2018) Advances in Diagnostics of Downy Mildews: Lessons Learned from Other Oomycetes and Future Challenges. Plant Disease 102:2, pages 265-275.
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Niloofar Vaghefi, Julie R. Kikkert, Melvin D. Bolton, Linda E. Hanson, Gary A. Secor, Scot C. Nelson & Sarah J. Pethybridge. (2017) Global genotype flow in Cercospora beticola populations confirmed through genotyping-by-sequencing. PLOS ONE 12:10, pages e0186488.
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S. WithersE. Gongora-CastilloD. GentA. ThomasP. S. OjiamboL. M. Quesada-Ocampo. (2016) Using Next-Generation Sequencing to Develop Molecular Diagnostics for Pseudoperonospora cubensis , the Cucurbit Downy Mildew Pathogen . Phytopathology® 106:10, pages 1105-1116.
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Zachariah R. Hansen, Brian J. Knaus, Javier F. Tabima, Caroline M. Press, Howard S. Judelson, Niklaus J. Grünwald & Christine D. Smart. (2016) SNP-Based Differentiation of Phytophthora infestans Clonal Lineages Using Locked Nucleic Acid Probes and High-Resolution Melt Analysis . Plant Disease 100:7, pages 1297-1306.
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Walter F. MahaffeeRob Stoll. (2016) The Ebb and Flow of Airborne Pathogens: Monitoring and Use in Disease Management Decisions. Phytopathology® 106:5, pages 420-431.
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Jeong Hwan Lee, Myong Ho Park & Sanghyeob Lee. (2016) Identification of Pseudoperonospora cubensis using real-time PCR and high resolution melting (HRM) analysis. Journal of General Plant Pathology 82:2, pages 110-115.
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