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Original Articles

Selection of a monoclonal antibody to detect PVYN and its use in ELISA and DIBA assays

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Pages 293-300 | Published online: 29 Dec 2009

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Read on this site (6)

Xianzhou Nie, Rudra P. Singh & Mathuresh Singh. (2004) Molecular and pathological characterization of N:O isolates of the Potato virus Y from Manitoba, Canada. Canadian Journal of Plant Pathology 26:4, pages 573-583.
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Rudra P. Singh, M. Singh & J.G. McDonald. (1998) Screening by a 3-primer PCR of North American PVYN isolates for European-type members of the tuber necrosis-inducing PVYNTN subgroup. Canadian Journal of Plant Pathology 20:3, pages 227-233.
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Mathuresh Singh & Rudra P. Singh. (1997) Potato virus Y detection: sensitivity of RT-PCR depends on the size of fragment amplified. Canadian Journal of Plant Pathology 19:2, pages 149-155.
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Mathuresh Singh & P. Singh. (1996) Nucleotide sequence and genome organization of a Canadian isolate of the common strain of potato virus Y (PVY°). Canadian Journal of Plant Pathology 18:3, pages 209-224.
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P. Ellis, R. Stace-Smith, G. Bowler & D.J. Mackenzie. (1996) Production of monoclonal antibodies for detection and identification of strains of potato virus Y. Canadian Journal of Plant Pathology 18:1, pages 64-70.
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M. Singh & R.P. Singh. (1995) Host dependent cross-protection between PVYN, PVY°, and PVA in potato cultivars and Solarium brachycarpum . Canadian Journal of Plant Pathology 17:1, pages 82-86.
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Articles from other publishers (32)

. (2022) Potato virus Y (potato mottle). CABI Compendium CABI Compendium.
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Jianhua Zhang, Xianzhou Nie, Upeksha Nanayakkara, Sébastien Boquel, Marie Andrée Giguère & Yvan Pelletier. (2013) Detection of P otato virus Y from the stylets of a single aphid by one‐step reverse transcription polymerase chain reaction . Entomologia Experimentalis et Applicata 147:1, pages 93-97.
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Matevž Rupar, Polona Kogovšek, Maruša Pompe-Novak, Ion Gutiérrez-Aguirre, Agnes Delaunay, Emmanuel Jacquot & Maja Ravnikar. (2013) Assessment of SNaPshot and single step RT-qPCR methods for discriminating Potato virus Y (PVY) subgroups. Journal of Virological Methods 189:1, pages 93-100.
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Ipsita Mallik, Nolan R. Anderson & Neil C. Gudmestad. (2012) Detection and Differentiation of Potato Virus Y Strains from Potato Using Immunocapture Multiplex RT-PCR. American Journal of Potato Research 89:3, pages 184-191.
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Gad Loebenstein & Victor Gaba. 2012. Viruses and Virus Diseases of Vegetables in the Mediterranean Basin. Viruses and Virus Diseases of Vegetables in the Mediterranean Basin 209 246 .
Stefania Rigotti, Carole Balmelli & Paul Gugerli. (2011) Census Report of the Potato Virus Y (PVY) Population in Swiss Seed Potato Production in 2003 and 2008. Potato Research 54:2, pages 105-117.
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Atefe Hosseini, Hossein Massumi, Jahangir Heydarnejad, Akbar Hosseini Pour & Arvind Varsani. (2010) Characterisation of potato virus Y isolates from Iran. Virus Genes 42:1, pages 128-140.
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Mohamad Chikh Ali, Tetsuo Maoka & Keiko T. Natsuaki. (2007) A point mutation changes the serotype of a Potato virus Y isolate; genomic determination of the serotype of PVY strains. Virus Genes 35:2, pages 359-367.
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Leonardo N. Fonseca, Alice K. Inoue-Nagata, Tatsuya Nagata, Rudra P. Singh & Antonio Carlos de Ávila. (2005) Diferenciação de estirpes de Potato virus Y (PVY) por RT-PCR. Horticultura Brasileira 23:4, pages 904-910.
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Emmanuel Jacquot, Michel Tribodet, Flora Croizat, Valérie Balme-Sinibaldi & Camille Kerlan. (2005) A single nucleotide polymorphism-based technique for specific characterization of YO and YN isolates of Potato virus Y (PVY).. Journal of Virological Methods 125:1, pages 83-93.
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R. P. Singh, D. L. McLaren, X. Nie & M. Singh. (2003) Possible Escape of a Recombinant Isolate of Potato virus Y by Serological Indexing and Methods of its Detection . Plant Disease 87:6, pages 679-685.
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F. Raboudi, A. Ben Moussa, H. Makni, M. Marrakchi & M. Makni. (2003) Serological detection of plant viruses in their aphid vectors and host plants in Tunisia. EPPO Bulletin 32:3, pages 495-498.
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H. Ounouna, C. Kerlan, P. Lafaye, M. J. Loukili & A. ElGaaied. (2002) Production of monoclonal antibodies against synthetic peptides of the N‐terminal region of Potato virus Y coat protein and their use in PVY strain differentiation . Plant Pathology 51:4, pages 487-494.
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Xianzhou Nie & Rudra P. Singh. (2002) A new approach for the simultaneous differentiation of biological and geographical strains of Potato virus Y by uniplex and multiplex RT-PCR. Journal of Virological Methods 104:1, pages 41-54.
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Xianzhou Nie & Rudra P. Singh. (2002) Probable geographical grouping of PVYN and PVYNTN based on sequence variation in P1 and 5′-UTR of PVY genome and methods for differentiating North American PVYNTN. Journal of Virological Methods 103:2, pages 145-156.
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Alan A Brunt. 2001. Virus and Virus-like Diseases of Potatoes and Production of Seed-Potatoes. Virus and Virus-like Diseases of Potatoes and Production of Seed-Potatoes 121 134 .
G. Boiteau, M. Singh, R. P. Singh, G. C. C. Tai & T. R. Turner. (1998) Rate of spread of PVYn by alateMyzus persicae (Sulzer) from infected to healthy plants under laboratory conditions. Potato Research 41:4, pages 335-344.
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H Weilguny & R.P Singh. (1998) Separation of Slovenian isolates of PVYNTN from the North American isolates of PVYN by a 3-primer PCR. Journal of Virological Methods 71:1, pages 57-68.
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Rudra P. Singh & Mathuresh Singh. (1998) Specific Detection of Potato Virus A in Dormant Tubers by Reverse-Transcription Polymerase Chain Reaction. Plant Disease 82:2, pages 230-234.
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D Iannelli, L D'Apice, C Cottone, M Viscardi, F Scala, A Zoina, G Del Sorbo, P Spigno & R Capparelli. (1997) Simultaneous detection of cucumber mosaic virus, tomato mosaic virus and potato virus Y by flow cytometry. Journal of Virological Methods 69:1-2, pages 137-145.
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A. K. Dhar & R. P. Singh. (1997) Molecular characterization of coat protein genes of serologically distinct isolates of potato virus Y necrotic strain. Canadian Journal of Microbiology 43:7, pages 677-683.
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N. R. Watts, M. Singh & R. P. Singh. (1997) Potato virus X isolates from potato collected in eastern Canada with different symptoms in tobacco differ in their coat proteins. American Potato Journal 74:4, pages 245-253.
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P. Ellis, R. Stace-Smith & G. de Villiers. (1997) Identification and Geographic Distribution of Serotypes of Potato Virus Y. Plant Disease 81:5, pages 481-484.
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L. Glais, C. Kerlan, M. Tribodet, V. Marie -Jeanne Tordo, C. Robaglia & S. Astier-Manifacier. (1996) Molecular characterization of potato virus YN isolates by PCR-RFLP. European Journal of Plant Pathology 102:7, pages 655-662.
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J. G. McDonald & R. P. Singh. (1996) Host range, symptomology, and serology of isolates of potato virus Y (PVY) that share properties with both the PVYN and PVYO strain groups. American Potato Journal 73:7, pages 309-315.
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Mathuresh Singh & Rudra P. Singh. (1996) Factors affecting detection of PVY in dormant tubers by reverse transcription polymerase chain reaction and nucleic acid spot hybridization. Journal of Virological Methods 60:1, pages 47-57.
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R. P. Singh, A. Boucher, T. H. Somerville & S. Coleman. (1996) Detection of potato viruses A, M, S, X, Yand leafroll and potato spindle tuber viroid from tissue culture plantlets using single leaf discs. American Potato Journal 73:3, pages 101-112.
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Mathuresh Singh & Rudra P. Singh. (1995) Digoxigenin-labelled cDNA probes for the detection of potato virus Y in dormant potato tubers. Journal of Virological Methods 52:1-2, pages 133-143.
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A.K. Dhar & R.P. Singh. (1994) Improvement in the sensitivity of PVYN detection by increasing the cDNA probe size. Journal of Virological Methods 50:1-3, pages 197-210.
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M. Singh, R. P. Singh & T. H. Somerville. (1994) Evaluation of tuber-bearingSolanum species for symptomology, as diagnostic hosts, and sources of immunityto potato virus Y necrotic strain (PVYN). American Potato Journal 71:9, pages 567-579.
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J. P. T. Valkonen, S. A. Slack & R. L. Plaisted. (1994) Use of the virus strain group concept to characterize the resistance to PVX and PVYo in the potato cv Allegany. American Potato Journal 71:8, pages 507-516.
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J. G. McDonald, G. T. Kristjansson, R. P. Singh, P. J. Ellis & W. B. McNab. (1994) Consecutive elisa screening with monoclonal antibodies to detect potato virus YN. American Potato Journal 71:3, pages 175-183.
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