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Technical Note

DNA Double-strand Break Analysis by CHEF Electrophoresis

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Pages 23-34 | Received 20 Nov 1989, Accepted 29 Jan 1990, Published online: 03 Jul 2009

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Read on this site (18)

Alexandra Jensen, Jürgen Debus & Klaus-Josef Weber. (2008) S-phase cell-specific modification by gemcitabine of PFGE-analyzed radiation-induced DNA fragmentation and rejoining. International Journal of Radiation Biology 84:9, pages 770-777.
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D. M. Smith & G. P. Raaphorst. (2003) Adaptive responses in human glioma cells assessed by clonogenic survival and DNA strand break analysis. International Journal of Radiation Biology 79:5, pages 333-339.
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Spiros Vamvakas, Esther H. Vock & Werner K. Lutz. (1997) On the Role of DNA Double-Strand Breaks in Toxicity and Carcinogenesis. Critical Reviews in Toxicology 27:2, pages 155-174.
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N. FORAY. (1996) Dose-rate effect on radiation-induced DNA double-strand breaks in the human fibroblast HF19 cell line. International Journal of Radiation Biology 69:2, pages 241-249.
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D. LATZ, W. C. DEWEY, M. FLENTJE, F. LOHR, F. WENZ and K. J. WEBER. (1996) Migration patterns in pulsed-field electrophoresis of DNA restriction fragments from log-phase mammalian cells after irradiation and incubation for repair. International Journal of Radiation Biology 70:6, pages 637-646.
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J. Dahm-Daphi & E. Dikomey. (1995) Separation of DNA Fragments Induced by Ionizing Irradiation Using a Graded-field Gel Electrophoresis. International Journal of Radiation Biology 67:2, pages 161-168.
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J. Wang, D.A. Delgado & D.F. Deen. (1995) Use of Pulsed-field Gel Electrophoresis to Investigate Factors Influencing the Measurement of DNA Double-strand Breaks in Human Brain Tumour Specimens. International Journal of Radiation Biology 67:2, pages 153-160.
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M. Flentje, B. Asadpour, D. Latz & K.J. Weber. (1993) Sensitivity of Neutral Filter Elution but Not PFGE Can Be Modified by Non-dsb Chromatin Damage. International Journal of Radiation Biology 63:6, pages 715-724.
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M. Rosemann, B. Kanon, A.W.T. Konings & H.H. Kampinga. (1993) An Image Analysis Technique for Detection of Radiation-induced DNA Fragmentation after CHEF Electrophoresis. International Journal of Radiation Biology 64:2, pages 245-249.
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K.J. Weber & M. Flentje. (1993) Lethality of Heavy Ion-induced DNA Double-strand Breaks in Mammalian Cells. International Journal of Radiation Biology 64:2, pages 169-178.
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M.D. Story, K.C. Garrett, P.J. Tofilon & R.E. Meyn. (1993) Influence of Irradiation Conditions on the Measurement of DNA Double-strand Breaks by Pulsed-field Gel Electrophoresis. International Journal of Radiation Biology 63:3, pages 297-304.
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A.A. Friedl, W. Beisker, K. Hahn, F. Eckardt-Schupp & A.M. Kellerer. (1993) Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules. International Journal of Radiation Biology 63:2, pages 173-181.
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S.J. Whitaker & T.J. McMillan. (1992) Oxygen Effect for DNA Double-strand Break Induction Determined by Pulsed-field Gel Electrophoresis. International Journal of Radiation Biology 61:1, pages 29-41.
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Articles from other publishers (22)

Satyendra K. Singh, Weizhong Wu, Wenqi Wu, Minli Wang & George Iliakis. (2009) Extensive Repair of DNA Double-Strand Breaks in Cells Deficient in the DNA-PK-Dependent Pathway of NHEJ after Exclusion of Heat-Labile Sites. Radiation Research 172:2, pages 152.
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Karin H. Karlsson, Irina Radulescu, Björn Rydberg & Bo Stenerlöw. (2008) Repair of Radiation-Induced Heat-Labile Sites is Independent of DNA-PKcs, XRCC1 and PARP. Radiation Research 169:5, pages 506-512.
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Natalia S. Zhdanova, Julia M. Minina, Tatjana V. Karamisheva, Irena Draskovic, Nikolai B. Rubtsov & Jose-Arturo Londoño-Vallejo. (2007) The very long telomeres in Sorex granarius (Soricidae, Eulipothyphla) contain ribosomal DNA. Chromosome Research 15:7, pages 881-890.
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Iwona Grądzka & Teresa Iwaneńko. (2005) A non-radioactive, PFGE-based assay for low levels of DNA double-strand breaks in mammalian cells. DNA Repair 4:10, pages 1129-1139.
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Yuichiro Yokota, Naoya Shikazono, Atsushi Tanaka, Yoshihiro Hase, Tomoo Funayama, Seiichi Wada & Masayoshi Inoue. (2005) Comparative Radiation Tolerance Based on the Induction of DNA Double-Strand Breaks in Tobacco BY-2 Cells and CHO-K1 Cells Irradiated with Gamma Rays. Radiation Research 163:5, pages 520-525.
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Hiroaki Terato & Hiroshi Ide. (2004) Clustered DNA damage induced by heavy ion particles. Biological Sciences in Space 18:4, pages 206-215.
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Bo Stenerlöw, Karin H. Karlsson, Brian Cooper & Björn Rydberg. (2003) Measurement of Prompt DNA Double-Strand Breaks in Mammalian Cells without Including Heat-Labile Sites: Results for Cells Deficient in Nonhomologous End Joining. Radiation Research 159:4, pages 502-510.
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Björn Rydberg. (2000) Radiation-Induced Heat-Labile Sites that Convert into DNA Double-Strand Breaks. Radiation Research 153:6, pages 805-812.
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N. Foray, C.F. Arlett & E.P. Malaise. (1997) Radiation-induced DNA double-strand breaks and the radiosensitivity of human cells: A closer look. Biochimie 79:9-10, pages 567-575.
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Letizia Palomba, Liliana Brambilla, Giorgio Brandi, Piero Sestili, Flaminio Cattabeni & Orazio Cantoni. (1996) Low levels of hydrogen peroxide and l-histidine induce DNA double-strand breakage and apoptosis. European Journal of Pharmacology 318:1, pages 167-173.
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G. Taucher-Scholz, J. Heilmann & G. Kraft. (1996) Induction of DNA strand breaks by heavy ions. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 107:1-4, pages 318-322.
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Piero Sestili, Flaminio Cattabeni & Orazio Cantoni. (1995) The L-histidine-mediated enhancement of hydrogen peroxide-induced DNA double strand breakage and cytotoxicity does not involve metabolic processes. Biochemical Pharmacology 50:11, pages 1823-1830.
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N. Foray, C.F. Arlett & E.P. Malaise. (1995) Dose-rate effect on induction and repair rate of radiation-induced DNA double-strand breaks in a normal and an ataxia telangiectasia human fibroblast cell line. Biochimie 77:11, pages 900-905.
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Reinhard Wurm, Neil G. Burnet, Nisha Duggal, John R. Yarnold & John H. Peacock. (1994) Cellular radiosensitivity and dna damage in primary human fibroblasts. International Journal of Radiation Oncology*Biology*Physics 30:3, pages 625-633.
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M. Schneider, G. Taucher-Scholz, J. Heilmann & G. Kraft. (1994) Combination of static-field gel electrophoresis and densitometric scanning for the determination of radiation-induced DNA double-strand breaks in CHO cells. Radiation and Environmental Biophysics 33:2, pages 111-124.
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Ulrich Hagen. (1994) Mechanisms of induction and repair of DNA double-strand breaks by ionizing radiation: Some contradictions. Radiation and Environmental Biophysics 33:1, pages 45-61.
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Herman C. H. Schoenmakers, José J. M. van der Meulen-Muisers & Maarten Koornneef. (1994) Asymmetric fusion between protoplasts of tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts of potato (Solanum tuberosum L.): the effects of gamma irradiation. Molecular and General Genetics MGG 242:3, pages 313-320.
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Lambert N. Daniel, Yan Mao & Saffiotti Umberto. (1993) Oxidative DNA damage by crystalline silica. Free Radical Biology and Medicine 14:5, pages 463-472.
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. 1993. Pulsed Field Gel Electrophoresis. Pulsed Field Gel Electrophoresis 193 244 .
William G. DeGraff, Murali C. Krishna, Dwight Kaufman & James B. Mitchell. (1992) Nitroxide-mediated protection against X-ray-and neocarzinostatin-induced DNA damage. Free Radical Biology and Medicine 13:5, pages 479-487.
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P.L. Olive, D. Wlodek, R.E. Durand & J.P. Banáth. (1992) Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Experimental Cell Research 198:2, pages 259-267.
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Michael C. Elia, John G. DeLuca & Matthews O. Bradley. (1991) Significance and measurement of DNA double strand breaks in mammalian cells. Pharmacology & Therapeutics 51:3, pages 291-327.
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