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Original Article

The Comet Moment as a Measure of DNA Damage in the Comet Assay

, , &
Pages 655-660 | Received 15 Sep 1994, Accepted 20 Feb 1995, Published online: 03 Jul 2009

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W. BOCKER, T. BAUCH, W.-U. MULLER and C. STREFFER. (1997) Technical Report Image analysis of comet assay measurements. International Journal of Radiation Biology 72:4, pages 449-460.
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I. Lagroye, B. Wettring, E. G. Moros, W. L. Straube, W. F. Pickard & J. L. Roti Roti. 2002. Wireless Phones And Health II. Wireless Phones And Health II 135 142 .
Joseph L. Roti Roti, Robert S. Malyapa & Eric Ahern. 1999. Wireless Phones and Health. Wireless Phones and Health 113 125 .
Lori A. HazlehurstNikola ValkovLee WisnerJonathan A. StoreyDavid BoulwareDaniel M. SullivanWilliam S. Dalton. (2001) Reduction in drug-induced DNA double-strand breaks associated with β1 integrin–mediated adhesion correlates with drug resistance in U937 cells. Blood 98:6, pages 1897-1903.
Crossref
Tsu-Shing Wang, Tsung-Yang Hsu, Chiao-Hui Chung, Alexander S.S Wang, Da-Tian Bau & Kun-Yan Jan. (2001) Arsenite induces oxidative DNA adducts and DNA-protein cross-links in mammalian cells. Free Radical Biology and Medicine 31:3, pages 321-330.
Crossref
Rita S. Raman, Philip J. Chan, Johannah U. Corselli, William C. Patton, John D. Jacobson, Steven R. Chan & Alan King. (2001) Comet assay of cumulus cell DNA status and the relationship to oocyte fertilization via intracytoplasmic sperm injection*. Human Reproduction 16:5, pages 831-835.
Crossref
Ramesh C. Chaubey, Hari N. Bhilwade, Rema Rajagopalan & Sanjay V. Bannur. (2001) Gamma ray induced DNA damage in human and mouse leucocytes measured by SCGE-Pro: a software developed for automated image analysis and data processing for Comet assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 490:2, pages 187-197.
Crossref
William D. Wright, Isabelle Lagroye, Peng Zhang, Robert S. Malyapa & Joseph L. Roti Roti. 2001. Cytometry: Part B. Cytometry: Part B 251 268 .
Makoto Naoi, Wakako Maruyama, Yukihito Akao, Jin Zhang & Hasan Parvez. (2000) Apoptosis induced by an endogenous neurotoxin, N-methyl(R)salsolinol, in dopamine neurons. Toxicology 153:1-3, pages 123-141.
Crossref
Georg Sauer, Klaus J. Weber, Peter Peschke & Michael J. Eble. (2000) Measurement of Hypoxia Using the Comet Assay Correlates with Preirradiation Microelectrode p O 2 Histography in R3327-AT Rodent Tumors . Radiation Research 154:4, pages 439-446.
Crossref
J.L He, W.L Chen, L.F Jin & H.Y Jin. (2000) Comparative evaluation of the in vitro micronucleus test and the comet assay for the detection of genotoxic effects of X-ray radiation. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 469:2, pages 223-231.
Crossref
E.Kristine Steele, Neil McClure & Sheena E.M Lewis. (2000) Comparison of the effects of two methods of cryopreservation on testicular sperm DNA. Fertility and Sterility 74:3, pages 450-453.
Crossref
Ulrich Glück & Jan‐Olaf Gebbers. (2009) The Comet Assay of Nasal Epithelia: Measurement of DNA Damage for the Assessment of Genotoxic Air Pollution. The Laryngoscope 110:1, pages 123-125.
Crossref
Fount Liu & Kun-Yan Jan. (2000) DNA damage in arsenite- and cadmium-treated bovine aortic endothelial cells. Free Radical Biology and Medicine 28:1, pages 55-63.
Crossref
Jessica E. Sutherland & Max Costa. (1999) Assays for DNA Damage . Current Protocols in Toxicology 2:1.
Crossref
Shugene Lynn, Ghi-Ling Yu & Kun Yan. (1999) Vicinal-Thiol-Containing Molecules Enhance but Mono-Thiol-Containing Molecules Reduce Nickel-Induced DNA Strand Breaks. Toxicology and Applied Pharmacology 160:2, pages 198-205.
Crossref
E Rojas, M.C Lopez & M Valverde. (1999) Single cell gel electrophoresis assay: methodology and applications. Journal of Chromatography B: Biomedical Sciences and Applications 722:1-2, pages 225-254.
Crossref
Stylianos M. Piperakis, Evangelos-E. Visvardis & Aspasia M. Tassiou. 1999. Oxidants and Antioxidants Part B. Oxidants and Antioxidants Part B 184 194 .
Shugene Lynn, Jaw-Nan Shiung, Jia-Ran Gurr & K.Y. Jan. (1998) Arsenite Stimulates Poly(ADP-Ribosylation) by Generation of Nitric Oxide. Free Radical Biology and Medicine 24:3, pages 442-449.
Crossref
Eckhard Bauer, Rolf-Dieter Recknagel, Ursula Fiedler, Leo Wollweber, Claudia Bock & Karl Otto Greulich. (1998) The distribution of the tail moments in single cell gel electrophoresis (comet assay) obeys a chi-square (χ2) not a gaussian distribution. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 398:1-2, pages 101-110.
Crossref
C. Harrison, J.D. Pearson, R.F. Bilton, D.R. Burton & J. Roberts. (1998) The comet moment ratio and other parameters obtained by applying image processing techniques and feature extraction to the SCGE assay. The comet moment ratio and other parameters obtained by applying image processing techniques and feature extraction to the SCGE assay.
Shugene Lynn, Hsien-Tsung Lai, Sou-Mang Kao, June Lai & K.Y. Jan. (1997) Cadmium Inhibits DNA Strand Break Rejoining in Methyl Methanesulfonate-Treated CHO-K1 Cells. Toxicology and Applied Pharmacology 144:1, pages 171-176.
Crossref
H Cerda, H Delincée, H Haine & H Rupp. (1997) The DNA `comet assay' as a rapid screening technique to control irradiated food. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 375:2, pages 167-181.
Crossref
J.E Yendle, H Tinwell, B.M Elliott & J Ashby. (1997) The genetic toxicity of time: Importance of DNA-unwinding time to the outcome of single-cell gel electrophoresis assays. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 375:2, pages 125-136.
Crossref

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