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Research Article

Solubilization of high affinity G-protein-coupled serotonin 1A receptors from bovine hippocampus using pre-micellar CHAPS at low concentration

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Pages 211-220 | Published online: 09 Jul 2009

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Read on this site (3)

Debanjana Ghosh & Nitin Chattopadhyay. (2013) Equilibrium and dynamic effects on ligand binding to biomacromolecules and biomimetic model systems. International Reviews in Physical Chemistry 32:3, pages 435-466.
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Yamuna Devi Paila, Thomas J. Pucadyil & Amitabha Chattopadhyay. (2005) The cholesterol-complexing agent digitonin modulates ligand binding of the bovine hippocampal serotonin1A receptor. Molecular Membrane Biology 22:3, pages 241-249.
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Vaishali Javadekar-Subhedar & Amitabha Chattopadhyay. (2004) Temperature-dependent interaction of the bovine hippocampal serotonin1A receptor with G-proteins. Molecular Membrane Biology 21:2, pages 119-123.
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Articles from other publishers (31)

Parijat Sarkar & Amitabha Chattopadhyay. (2017) Solubilization of the serotonin 1A receptor monitored utilizing membrane dipole potential. Chemistry and Physics of Lipids 209, pages 54-60.
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Parijat Sarkar & Amitabha Chattopadhyay. (2015) Dipolar rearrangement during micellization explored using a potential-sensitive fluorescent probe. Chemistry and Physics of Lipids 191, pages 91-95.
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Amitabha Chattopadhyay, Bhagyashree D. Rao & Md. Jafurulla. 2015. Membrane Proteins?Engineering, Purification and Crystallization. Membrane Proteins?Engineering, Purification and Crystallization 117 134 .
Christel Logez, Sylvie Berger, Céline Legros, Jean-Louis Banères, William Cohen, Philippe Delagrange, Olivier Nosjean, Jean A. Boutin, Gilles Ferry, Frédéric Simonin & Renaud Wagner. (2014) Recombinant Human Melatonin Receptor MT1 Isolated in Mixed Detergents Shows Pharmacology Similar to That in Mammalian Cell Membranes. PLoS ONE 9:6, pages e100616.
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Franck Talmont, Lionel Moul?dous, Catherine Mollereau & Jean-Marie Zajac. (2014) Solubilization and reconstitution of the mu-opioid receptor expressed in human neuronal SH-SY5Y and CHO cells. Peptides 55, pages 79-84.
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Md. Jafurulla, Bhagyashree D. Rao, Sugunan Sreedevi, Jean-Marie Ruysschaert, Douglas F. Covey & Amitabha Chattopadhyay. (2014) Stereospecific requirement of cholesterol in the function of the serotonin1A receptor. Biochimica et Biophysica Acta (BBA) - Biomembranes 1838:1, pages 158-163.
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Pil Seok Chae, Aiman Sadaf & Samuel H. Gellman. (2013) Hydrophobic Variations of N ‐Oxide Amphiphiles for Membrane Protein Manipulation: Importance of Non‐hydrocarbon Groups in the Hydrophobic Portion . Chemistry – An Asian Journal 9:1, pages 110-116.
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Arunima Chaudhuri, Sourav Haldar & Amitabha Chattopadhyay. (2012) Structural transition in micelles: novel insight into microenvironmental changes in polarity and dynamics. Chemistry and Physics of Lipids 165:4, pages 497-504.
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Pushpendra Singh, Md. Jafurulla, Yamuna Devi Paila & Amitabha Chattopadhyay. (2011) Desmosterol replaces cholesterol for ligand binding function of the serotonin1A receptor in solubilized hippocampal membranes: Support for nonannular binding sites for cholesterol?. Biochimica et Biophysica Acta (BBA) - Biomembranes 1808:10, pages 2428-2434.
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Deboleena Sarkar, Debanjana Ghosh, Paramita Das & Nitin Chattopadhyay. (2010) Electrostatic Pushing Effect: A Prospective Strategy for Enhanced Drug Delivery. The Journal of Physical Chemistry B 114:39, pages 12541-12548.
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Gabriela Maria Pavan de Arruda Camargo, Luiz Antônio de Arruda Camargo & Wilson Abrão Saad. (2010) Role of serotonergic 5-HT1A and oxytocinergic receptors of the lateral septal area in sodium intake regulation. Behavioural Brain Research 209:2, pages 260-266.
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Arunima Chaudhuri, Sourav Haldar & Amitabha Chattopadhyay. (2009) Organization and dynamics in micellar structural transition monitored by pyrene fluorescence. Biochemical and Biophysical Research Communications 390:3, pages 728-732.
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Patrícia de Souza Villa, José Vanderlei Menani, Gabriela Maria Pavan de Arruda Camargo, Luiz Antônio de Arruda Camargo & Wilson Abrão Saad. (2008) Activation of the serotonergic 5-HT1A receptor in the paraventricular nucleus of the hypothalamus inhibits water intake and increases urinary excretion in water-deprived rats. Regulatory Peptides 150:1-3, pages 14-20.
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Md. Jafurulla & Amitabha Chattopadhyay. (2007) Solubilization of human serotonin1A receptors expressed in neuronal cells. Chemistry and Physics of Lipids 150:2, pages 244-249.
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Shanti Kalipatnapu & Amitabha Chattopadhyay. (2007) Membrane Organization and Function of the Serotonin1A Receptor. Cellular and Molecular Neurobiology 27:8, pages 1097-1116.
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Amitabha Chattopadhyay, Yamuna Devi Paila, Md. Jafurulla, Arunima Chaudhuri, Pushpendra Singh, M.R.V.S. Murty & M. Vairamani. (2007) Differential effects of cholesterol and 7-dehydrocholesterol on ligand binding of solubilized hippocampal serotonin1A receptors: Implications in SLOS. Biochemical and Biophysical Research Communications 363:3, pages 800-805.
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Mohamed Elmogy, Masafumi Iwami & Sho Sakurai. (2007) Solubilization of the Ecdysone Binding Protein from Anterior Silk Gland Cell Membranes of the Silkworm, Bombyx mori. Zoological Science 24:10, pages 971-977.
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Soumi Mukherjee, Mamata Kombrabail, G. Krishnamoorthy & Amitabha Chattopadhyay. (2007) Dynamics and heterogeneity of bovine hippocampal membranes: Role of cholesterol and proteins. Biochimica et Biophysica Acta (BBA) - Biomembranes 1768:9, pages 2130-2144.
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Thomas J. Pucadyil & Amitabha Chattopadhyay. (2006) The human serotonin1A receptor exhibits G-protein-dependent cell surface dynamics. Glycoconjugate Journal 24:1, pages 25-31.
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Thomas J. Pucadyil & Amitabha Chattopadhyay. (2006) Role of cholesterol in the function and organization of G-protein coupled receptors. Progress in Lipid Research 45:4, pages 295-333.
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Satinder S. Rawat, Devaki A. Kelkar & Amitabha Chattopadhyay. (2005) Effect of Structural Transition of the Host Assembly on Dynamics of an Ion Channel Peptide: A Fluorescence Approach. Biophysical Journal 89:5, pages 3049-3058.
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Soumi Mukherjee & Amitabha Chattopadhyay. (2005) Monitoring the organization and dynamics of bovine hippocampal membranes utilizing Laurdan generalized polarization. Biochimica et Biophysica Acta (BBA) - Biomembranes 1714:1, pages 43-55.
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Shanti Kalipatnapu & Amitabha Chattopadhyay. (2005) Membrane Protein Solubilization: Recent Advances and Challenges in Solubilization of Serotonin1A Receptors. IUBMB Life (International Union of Biochemistry and Molecular Biology: Life) 57:7, pages 505-512.
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Thomas J. Pucadyil, Shanti Kalipatnapu & Amitabha Chattopadhyay. (2005) The Serotonin1A A Receptor: A Representative Member of the Serotonin Receptor Family. Cellular and Molecular Neurobiology 25:3-4, pages 553-580.
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Amitabha Chattopadhyay, Md. Jafurulla, Shanti Kalipatnapu, Thomas J. Pucadyil & Kaleeckal G. Harikumar. (2005) Role of cholesterol in ligand binding and G-protein coupling of serotonin1A receptors solubilized from bovine hippocampus. Biochemical and Biophysical Research Communications 327:4, pages 1036-1041.
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Shanti Kalipatnapu & Amitabha Chattopadhyay. (2004) A GFP fluorescence-based approach to determine detergent insolubility of the human serotonin 1A receptor . FEBS Letters 576:3, pages 455-460.
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Shanti Kalipatnapu, Md. Jafurulla, Nandhini Chandrasekaran & Amitabha Chattopadhyay. (2004) Effect of Mg2+ on guanine nucleotide sensitivity of ligand binding to serotonin1A receptors from bovine hippocampus. Biochemical and Biophysical Research Communications 323:2, pages 372-376.
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Thomas J. Pucadyil, Sandeep Shrivastava & Amitabha Chattopadhyay. (2004) The sterol-binding antibiotic nystatin differentially modulates ligand binding of the bovine hippocampal serotonin1A receptor. Biochemical and Biophysical Research Communications 320:2, pages 557-562.
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Thomas J Pucadyil & Amitabha Chattopadhyay. (2004) Cholesterol modulates ligand binding and G-protein coupling to serotonin1A receptors from bovine hippocampus. Biochimica et Biophysica Acta (BBA) - Biomembranes 1663:1-2, pages 188-200.
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H. Raghuraman, Suman K. Pradhan & Amitabha Chattopadhyay. (2004) Effect of Urea on the Organization and Dynamics of Triton X-100 Micelles:  A Fluorescence Approach. The Journal of Physical Chemistry B 108:7, pages 2489-2496.
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Thomas J Pucadyil & Amitabha Chattopadhyay. (2004) Exploring detergent insolubility in bovine hippocampal membranes: a critical assessment of the requirement for cholesterol. Biochimica et Biophysica Acta (BBA) - Biomembranes 1661:1, pages 9-17.
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