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Molecular Membrane Biology Volume 25, 2008 - Issue 6-7
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Expression, purification and activities of the entire family of intact membrane sensor kinases from Enterococcus faecalis

Pikyee Ma Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Pikyee Ma Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Hayley M. Yuille Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Pikyee Ma Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Hayley M. Yuille Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Victor Blessie Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Nadine Göhring Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Zsófia Iglói Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
,
Kenzo Nishiguchi Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Jiro Nakayama Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Peter J. F. Henderson Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK
&
Mary K. Phillips-Jones Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UKCorrespondence[email protected]
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Pages 449-473 | Received 15 May 2008, Published online: 09 Jul 2009

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Kim E. Bettaney, Preethi Sukumar, Rohanah Hussain, Giuliano Siligardi, Peter J. F. Henderson & Simon G. Patching. (2013) A systematic approach to the amplified expression, functional characterization and purification of inositol transporters from Bacillus subtilis . Molecular Membrane Biology 30:1, pages 3-14.
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Articles from other publishers (26)

Mikhail Bogdanov. 2024. Bacterial Secretion Systems. Bacterial Secretion Systems 159 180 .
Junqi Zhang, Rong Fang, Qi Peng, Shizhou Wu & Lei Lei. (2023) The Regulations of Essential WalRK Two-Component System on Enterococcus faecalis. Journal of Clinical Medicine 12:3, pages 767.
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Brenna J. C. Walsh, Sofia Soares Costa, Katherine A. Edmonds, Jonathan C. Trinidad, Federico M. Issoglio, José A. Brito & David P. Giedroc. (2022) Metabolic and Structural Insights into Hydrogen Sulfide Mis-Regulation in Enterococcus faecalis. Antioxidants 11:8, pages 1607.
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Pikyee Ma & Mary K. Phillips-Jones. (2021) Membrane Sensor Histidine Kinases: Insights from Structural, Ligand and Inhibitor Studies of Full-Length Proteins and Signalling Domains for Antibiotic Discovery. Molecules 26:16, pages 5110.
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E.-L. Jeong, S.J. Broad, R.G. Moody & M.K. Phillips-Jones. (2020) The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer Rhodobacter sphaeroides is regulated by the global Prr pathway. International Journal of Hydrogen Energy 45:51, pages 26840-26854.
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Sean Littlewood, Helena Tattersall, Charlotte S. Hughes, Rohanah Hussain, Pikyee Ma, Stephen E. Harding, Jiro Nakayama & Mary K. Phillips‐Jones. (2019) The gelatinase biosynthesis‐activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing . FEBS Letters 594:3, pages 553-563.
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Liying Zhang, Chunshan Quan, Xuning Zhang, Wen Xiong & Shengdi Fan. (2019) Proteoliposome‐based model for screening inhibitors targeting histidine kinase AgrC. Chemical Biology & Drug Design 93:5, pages 712-723.
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Giuliano SiligardiCharlotte S. HughesRohanah Hussain. (2018) Characterisation of sensor kinase by CD spectroscopy: golden rules and tips. Biochemical Society Transactions 46:6, pages 1627-1642.
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Aalishaa A. Azam, Jean M. Kinder, G. Nasir Khan, Ade Alase, Pikyee Ma, Yang Liu, James R. Ault, Peter J. F. Henderson, Babur Z. Chowdhry, Bruce D. Alexander, Stephen E. Harding & Mary K. Phillips-Jones. (2018) Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions. European Biophysics Journal 47:7, pages 723-737.
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Marvin V. Dilworth, Mathilde S. Piel, Kim E. Bettaney, Pikyee Ma, Ji Luo, David Sharples, David R. Poyner, Stephane R. Gross, Karine Moncoq, Peter J.F. Henderson, Bruno Miroux & Roslyn M. Bill. (2018) Microbial expression systems for membrane proteins. Methods 147, pages 3-39.
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Irshad Ahmad, Nighat Nawaz, Nizam M. Darwesh, Sadeeq ur Rahman, Mohammad Z. Mustafa, Sher B. Khan & Simon G. Patching. (2018) Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli. Protein Expression and Purification 144, pages 12-18.
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C.S. Hughes, E. Longo, M.K. Phillips-Jones & R. Hussain. (2017) Characterisation of the selective binding of antibiotics vancomycin and teicoplanin by the VanS receptor regulating type A vancomycin resistance in the enterococci. Biochimica et Biophysica Acta (BBA) - General Subjects 1861:8, pages 1951-1959.
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Mary K. Phillips-Jones, Guy Channell, Claire J. Kelsall, Charlotte S. Hughes, Alison E. Ashcroft, Simon G. Patching, Vlad Dinu, Richard B. Gillis, Gary G. Adams & Stephen E. Harding. (2017) Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin. Scientific Reports 7:1.
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Moazur Rahman, Fouzia Ismat, Li Jiao, Jocelyn M. Baldwin, David J. Sharples, Stephen A. Baldwin & Simon G. Patching. (2016) Characterisation of the DAACS Family Escherichia coli Glutamate/Aspartate-Proton Symporter GltP Using Computational, Chemical, Biochemical and Biophysical Methods. The Journal of Membrane Biology 250:2, pages 145-162.
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Rohanah HussainStephen E. HardingCharlotte S. HughesPikyee MaSimon G. PatchingShalini EdaraGiuliano SiligardiPeter J.F. HendersonMary K. Phillips-Jones. (2016) Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochemical Society Transactions 44:3, pages 810-823.
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Pikyee Ma, Simon G. Patching, Ekaterina Ivanova, Jocelyn M. Baldwin, David Sharples, Stephen A. Baldwin & Peter J. F. Henderson. (2016) Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity. Microbiology 162:5, pages 823-836.
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Jennifer L. Dale, Julian Cagnazzo, Chi Q. Phan, Aaron M. T. Barnes & Gary M. Dunny. (2015) Multiple Roles for Enterococcus faecalis Glycosyltransferases in Biofilm-Associated Antibiotic Resistance, Cell Envelope Integrity, and Conjugative Transfer. Antimicrobial Agents and Chemotherapy 59:7, pages 4094-4105.
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Giuliano Siligardi, Rohanah Hussain, Simon G. Patching & Mary K. Phillips-Jones. (2014) Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy. Biochimica et Biophysica Acta (BBA) - Biomembranes 1838:1, pages 34-42.
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Lina Wang, Chunshan Quan, Baoquan Liu, Jianfeng Wang, Wen Xiong, Pengchao Zhao & Shengdi Fan. (2013) Functional Reconstitution of Staphylococcus aureus Truncated AgrC Histidine Kinase in a Model Membrane System. PLoS ONE 8:11, pages e80400.
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Pikyee Ma, Filipa Varela, Malgorzata Magoch, Ana Rita Silva, Ana Lúcia Rosário, José Brito, Tânia Filipa Oliveira, Przemyslaw Nogly, Miguel Pessanha, Meike Stelter, Arnulf Kletzin, Peter J. F. Henderson & Margarida Archer. (2013) An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli. PLoS ONE 8:10, pages e76913.
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Mary K. Phillips-Jones, Simon G. Patching, Shalini Edara, Jiro Nakayama, Rohanah Hussain & Giuliano Siligardi. (2013) Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptideinhibitor siamycin I revealed through synchrotron radiation circular dichroism. Phys. Chem. Chem. Phys. 15:2, pages 444-447.
Crossref
Simon G. Patching, Shalini Edara, Pikyee Ma, Jiro Nakayama, Rohanah Hussain, Giuliano Siligardi & Mary K. Phillips-Jones. (2012) Interactions of the intact FsrC membrane histidine kinase with its pheromone ligand GBAP revealed through synchrotron radiation circular dichroism. Biochimica et Biophysica Acta (BBA) - Biomembranes 1818:7, pages 1595-1602.
Crossref
Pikyee Ma, Kenzo Nishiguchi, Hayley M. Yuille, Lianne M. Davis, Jiro Nakayama & Mary K. Phillips-Jones. (2011) Anti-HIV siamycin I directly inhibits autophosphorylation activity of the bacterial FsrC quorum sensor and other ATP-dependent enzyme activities. FEBS Letters 585:17, pages 2660-2664.
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María Florencia Del Papa & Marta Perego. (2011) Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters . Journal of Bacteriology 193:7, pages 1527-1532.
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Alina D. Gutu, Kyle J. Wayne, Lok-To Sham & Malcolm E. Winkler. (2010) Kinetic Characterization of the WalRK Spn (VicRK) Two-Component System of Streptococcus pneumoniae : Dependence of WalK Spn (VicK) Phosphatase Activity on Its PAS Domain . Journal of Bacteriology 192:9, pages 2346-2358.
Crossref
Birgit E Scharf. (2010) Summary of useful methods for two-component system research. Current Opinion in Microbiology 13:2, pages 246-252.
Crossref

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