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Reliable scale-up of membrane protein over-expression by bacterial auto-induction: From microwell plates to pilot scale fermentations

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Pages 588-598 | Received 20 Sep 2008, Published online: 09 Jul 2009

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Xiaobing Xia, Vincent L. G. Postis, Moazur Rahman, Gareth S. A. Wright, Peter C. J. Roach, Sarah E. Deacon, Jean C. Ingram, Peter J. F. Henderson, John B. C. Findlay, Simon E. V. Phillips, Vincent L. G. Postis, Michael J. McPherson & Stephen A. Baldwin. (2008) Investigation of the structure and function of a Shewanella oneidensis arsenical-resistance family transporter. Molecular Membrane Biology 25:8, pages 691-701.
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F A Laksmi, I Nuryana, C Vivid & M I Hadi. (2023) Autoinduction expression of Bst DNA polymerase using lac operon-controlled expression systems in Escherichia coli. IOP Conference Series: Earth and Environmental Science 1255:1, pages 012050.
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Sophia A. H. Heyde & Morten H. H. Nørholm. (2021) Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity. Communications Biology 4:1.
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Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan & Nicholas Noinaj. 2021. Structure and Function of Membrane Proteins. Structure and Function of Membrane Proteins 101 136 .
Yixin Liu, Ana Pavić, Joshua T. Farley, Carine de Marcos Lousa, Adrian Goldman & Vincent L. G. Postis. 2020. Biophysics of Membrane Proteins. Biophysics of Membrane Proteins 3 49 .
J. Porter Hunt, Emily Long Zhao, Mehran Soltani, Madison Frei, J. Andrew D. Nelson & Bradley C. Bundy. (2019) Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase. Synthetic and Systems Biotechnology 4:4, pages 220-224.
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Andrea E. Rawlings. (2018) Membrane protein engineering to the rescue. Biochemical Society Transactions 46:6, pages 1541-1549.
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Marvin V. Dilworth, Mathilde S. Piel, Kim E. Bettaney, Pikyee Ma, Ji Luo, David Sharples, David R. Poyner, Stephane R. Gross, Karine Moncoq, Peter J.F. Henderson, Bruno Miroux & Roslyn M. Bill. (2018) Microbial expression systems for membrane proteins. Methods 147, pages 3-39.
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Irshad Ahmad, Nighat Nawaz, Nizam M. Darwesh, Sadeeq ur Rahman, Mohammad Z. Mustafa, Sher B. Khan & Simon G. Patching. (2018) Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli. Protein Expression and Purification 144, pages 12-18.
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Cheng Ma, Zhenyu Hao, Gerard Huysmans, Amelia Lesiuk, Per Bullough, Yingying Wang, Mark Bartlam, Simon E. Phillips, James D. Young, Adrian Goldman, Stephen A. Baldwin & Vincent L. G. Postis. (2015) A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters. PLOS ONE 10:11, pages e0143010.
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Daniele Araujo Pereira, Caio Abércioda Silva, Mario Augusto Ono, Odilon Vidotto & Marilda Carlos Vidotto. (2015) Humoral Immune Response of Immunized Sows with Recombinant Proteins of Enterotoxigenic <i>Escherichia coli</i>. World Journal of Vaccines 05:01, pages 60-68.
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Chiara Lee, Hae Joo Kang, Anna Hjelm, Abdul Aziz Qureshi, Emmanuel Nji, Hassanul Choudhury, Konstantinos Beis, Jan-Willem de Gier & David Drew. (2014) MemStar: A one‐shot Escherichia coli ‐based approach for high‐level bacterial membrane protein production . FEBS Letters 588:20, pages 3761-3769.
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Vincent G. L. Postis, Andrea E. Rawlings, Amelia Lesiuk & Stephen A. Baldwin. 2013. Ion Channels. Ion Channels 33 54 .
Gerard H. M. HuysmansNathan ChanJocelyn M. BaldwinVincent L. G. PostisSvetomir B. TzokovSarah E. DeaconSylvia Y. M. Yao, James D. Young, Michael J. McPhersonPer A. BulloughStephen A. Baldwin. (2012) A urea channel from Bacillus cereus reveals a novel hexameric structure . Biochemical Journal 445:2, pages 157-166.
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Massoud Saidijam, Kim E. Bettaney, Dong Leng, Pikyee Ma, Zhiqiang Xu, Jeffrey N. Keen, Nicholas G. Rutherford, Alison Ward, Peter J. F. Henderson, Gerda Szakonyi, Qinghu Ren, Ian T. Paulsen, Ingerid Nes, Jasmin K. Kroeger & Anne‐Brit Kolsto. 2011. Advances in Enzymology and Related Areas of Molecular Biology. Advances in Enzymology and Related Areas of Molecular Biology 147 166 .
Xiaole Wang, Helle Hald, Heidi A. Ernst, Jan Egebjerg, Kenneth V. Christensen, Michael Gajhede, Jette S. Kastrup & Osman Mirza. (2010) Over-expression, purification and characterization of an Asc-1 homologue from Gloeobacter violaceus. Protein Expression and Purification 71:2, pages 179-183.
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Christopher M.M. Koth & Jian Payandeh. 2009. 43 86 .

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